BTech
Course Structure and Syllabus for Biotechnology (To
be applicable from 2013 batch onwards) |
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Course
No. |
Course Name |
L |
T |
P |
C |
|
Course
No. |
Course Name |
L |
T |
P |
C |
Semester - 1 |
|
Semester -2 |
||||||||||
CH101 |
Chemistry |
3 |
1 |
0 |
8 |
|
BT101 |
Modern
Biology |
3 |
0 |
0 |
6 |
EE101 |
Electrical
Sciences |
3 |
1 |
0 |
8 |
|
CS
101 |
Introduction
to Computing |
3 |
0 |
0 |
6 |
MA101 |
Mathematics
- I |
3 |
1 |
0 |
8 |
|
MA102 |
Mathematics
- II |
3 |
1 |
0 |
8 |
PH101 |
Physics
- I |
2 |
1 |
0 |
6 |
|
ME101 |
Engineering
Mechanics |
3 |
1 |
0 |
8 |
CH110 |
Chemistry
Laboratory |
0 |
0 |
3 |
3 |
|
PH102 |
Physics
- II |
2 |
1 |
0 |
6 |
ME110/ PH 110 |
Workshop
/Physics Laboratory |
0 |
0 |
3 |
3 |
|
CS110 |
Computing
Laboratory |
0 |
0 |
3 |
3 |
ME
111 |
Engineering
Drawing |
1 |
0 |
3 |
5 |
|
EE102 |
Basic
Electronics Laboratory |
0 |
0 |
3 |
3 |
SA
101 |
Physical
Training -I |
0 |
0 |
2 |
0 |
|
PH110/ ME 110 |
Physics
Laboratory/Workshop |
0 |
0 |
3 |
3 |
|
SA
102 |
Physical
Training -II |
0 |
0 |
2 |
0 |
||||||
12 |
4 |
9 |
41 |
|
|
|||||||
|
|
|
14 |
3 |
9 |
43 |
||||||
Semester 3 |
|
Semester 4 |
||||||||||
MA201 |
Mathematics-
III |
3 |
1 |
0 |
8 |
|
BT202 |
Microbiology |
3 |
0 |
0 |
6 |
BT201 |
Biochemistry |
3 |
0 |
0 |
6 |
|
BT205 |
Biophysics |
2 |
1 |
0 |
6 |
CL201
|
Chemical
Process Calculations |
2 |
1 |
0 |
6 |
|
BT208 |
Molecular
Biology & Genetic Engineering |
3 |
1 |
0 |
8 |
CL202
|
Fluid
Mechanics |
3 |
1 |
0 |
8 |
|
CL205 |
Mass
Transfer Operation - I |
2 |
1 |
0 |
6 |
CL203
|
Chemical
Engineering Thermodynamics -I |
2 |
1 |
0 |
6 |
|
HS2xx |
HSS
Elective - II |
3 |
0 |
0 |
6 |
HS2xx |
HSS
Elective - I |
3 |
0 |
0 |
6 |
|
BT290 |
Biomolecular Analysis
Laboratory |
0 |
0 |
6 |
6 |
NCC/NSO/COS |
0 |
0 |
2 |
0 |
|
NCC/NSO/COS |
0 |
0 |
2 |
0 |
||
16 |
4 |
0 |
40 |
|
|
|
13 |
3 |
6 |
38 |
||
Semester 5 |
|
Semester 6 |
||||||||||
BT301 |
Bioinformatics
and Computational Biology |
2 |
0 |
0 |
4 |
|
BT302 |
Biochemical
Engineering |
3 |
1 |
0 |
8 |
BT303 |
Immunology |
3 |
0 |
0 |
6 |
|
BT305 |
Industrial
Microbiology |
3 |
0 |
0 |
6 |
BT309 |
Plant
Biotechnology |
3 |
0 |
0 |
6 |
|
BT306 |
IPR,
Ethics and Bio-safety |
3 |
0 |
0 |
6 |
CL303
|
Chemical
Reaction Engineering - I |
2 |
1 |
0 |
6 |
|
BT308 |
Animal
Cell Biotechnology |
3 |
0 |
0 |
6 |
HS3xx |
HSS
Elective -III |
3 |
0 |
0 |
6 |
|
BTxxx |
Departmental
Elective - I |
3 |
0 |
0 |
6 |
BT380 |
Molecular
Biotechnology Laboratory |
0 |
0 |
6 |
6 |
|
BT330 |
Biochemical
Engineering Laboratory |
0 |
0 |
6 |
6 |
BT310 |
Bioinformatics
and Computational Biology Lab |
0 |
0 |
6 |
6 |
|
|
|
15 |
1 |
6 |
38 |
13 |
1 |
12 |
40 |
|
|
|
|
|
|
|
||
Semester 7 |
|
Semester 8 |
||||||||||
BT404 |
Bioseparation Engineering |
3 |
0 |
0 |
6 |
|
BT401 |
Frontiers
in Biotechnology |
3 |
0 |
0 |
6 |
BT405 |
Environmental
Biotechnology |
3 |
0 |
0 |
6 |
|
BTxxx |
Departmental
Elective - IV |
3 |
0 |
0 |
6 |
BTxxx |
Departmental
Elective - II |
3 |
0 |
0 |
6 |
|
BTxxx |
Departmental
Elective - V |
3 |
0 |
0 |
6 |
BTxxx |
Departmental
Elective - III |
3 |
0 |
0 |
6 |
|
HS4xx |
HSS
Elective - IV |
3 |
0 |
0 |
6 |
XXxxx |
Open
Elective - I |
3 |
0 |
0 |
6 |
|
XXxxx |
Open
Elective - II |
3 |
0 |
0 |
6 |
BT
498 |
Project
-I |
0 |
0 |
6 |
6 |
|
BT
499 |
Project
-II |
0 |
0 |
6 |
6 |
15 |
0 |
6 |
36 |
|
|
|
15 |
0 |
6 |
36 |
CH 101 Chemistry (3-1-0-8) Structure
and Bonding; Origin of quantum theory, postulates of quantum mechanics;
Schrodinger wave equation: operators and observables, superposition theorem
and expectation values, solutions for particle in a box, harmonic oscillator,
rigid rotator, hydrogen atom; Selection rules of microwave and vibrational spectroscopy; Spectroscopic term symbol;
Molecular orbitals: LCAO-MO; Huckel
theory of conjugated systems; Rotational, vibrational
and electronic spectroscopy; Chemical Thermodynamics: The zeroth
and first law, Work, heat, energy and enthalpies; The relation between Cv and Cp; Second law:
entropy, free energy (the Helmholtz and Gibbs) and chemical potential; Third
law; Chemical equilibrium; Chemical kinetics: The rate of reaction,
elementary reaction and chain reaction; Surface: The properties of liquid
surface, surfactants, colloidal systems, solid surfaces, physisorption
and chemisorption; The periodic table of elements;
Shapes of inorganic compounds; Chemistry of materials; Coordination
compounds: ligand, nomenclature, isomerism,
stereochemistry, valence bond, crystal field and molecular orbital theories;
Bioinorganic chemistry and organometallic
chemistry; Stereo and regio-chemistry of organic
compounds, conformers; Pericyclic reactions;
Organic photochemistry; Bioorganic chemistry: Amino acids, peptides,
proteins, enzymes, carbohydrates, nucleic acids and lipids; Macromolecules
(polymers); Modern techniques in structural elucidation of compounds (UV-vis, IR, NMR); Solid phase synthesis and combinatorial
chemistry; Green chemical processes. Texts:
1. P. W. Atkins, Physical Chemistry, 5th Ed., ELBS, 1994. 2. C.
N. Banwell, and E. M. McCash,
Fundamentals of Molecular Spectroscopy,
4th Ed., Tata McGraw-Hill, 1962. 3. F.
A. Cotton, and G. Wilkinson, Advanced
Inorganic Chemistry, 3rd Ed., Wiley Eastern Ltd., New Delhi,
1972, reprint in 1988. 4. D. J. Shriver, P. W. Atkins, and C. H.
Langford, Inorganic Chemistry, 2nd
Ed., ELBS ,1994. 5. S. H. Pine, Organic Chemistry, McGraw-Hill, 5th Ed., 1987 References: 1. I. A. Levine, Physical Chemistry, 4th Ed., McGraw-Hill, 1995. 2. I. A. Levine, Quantum Chemistry, EE Ed., prentice Hall, 1994. 3. G. M. Barrow, Introduction to Molecular Spectroscopy, International Edition,
McGraw-Hill, 1962 4. J.
E. Huheey, E. A. Keiter
and R. L. Keiter, Inorganic Chemistry: Principle, structure and reactivity, 4th
Ed., Harper Collins, 1993 5. L. G. Wade (Jr.), Organic Chemistry, Prentice Hall, 1987. |
CS 101
Introduction to Computing (3-0-0-6)
Introduction:
The von Neumann architecture, machine language, assembly language, high level
programming languages, compiler, interpreter, loader, linker, text editors,
operating systems, flowchart; Basic features of programming (Using C): data
types, variables, operators,
expressions, statements, control structures, functions; Advanced
programming features: arrays and pointers, recursion, records (structures),
memory management, files, input/output, standard library functions,
programming tools, testing and debugging; Fundamental operations on data:
insert, delete, search, traverse and modify; Fundamental data structures:
arrays, stacks, queues, linked lists; Searching and sorting: linear search,
binary search, insertion-sort, bubble-sort, selection-sort, radix-sort, counting-sort;
Introduction to object-oriented programming Texts:
1. A Kelly and I Pohl, A Book on C, 4th Ed.,
Pearson Education, 1999. 2. A M Tenenbaum,
Y Langsam and M J Augenstein,
Data Structures Using C, Prentice
Hall India, 1996. References: 1.
H Schildt, C:
The Complete Reference, 4th Ed., Tata Mcgraw
Hill, 2000 2. B Kernighan and
D Ritchie, The C Programming Language,
4th Ed., Prentice Hall of India, 1988. |
CS 110 Computing
Laboratory (0-0-3-3)
Programming
Laboratory will be set in consonance with the material covered in CS101. This
will include assignments in a programming language like C. References: 1.
B. Gottfried and J. Chhabra, Programming With C,
Tata Mcgraw Hill, 2005 MA 102 Mathematics
- II (3-1-0-8) Vector functions of one variable –
continuity and differentiability; functions of several variables –
continuity, partial derivatives, directional derivatives, gradient,
differentiability, chain rule; tangent planes and normals,
maxima and minima, Lagrange multiplier method; repeated and multiple
integrals with applications to volume, surface area, moments of inertia,
change of variables; vector fields, line and surface integrals;
Green’s, Gauss’ and Stokes’ theorems and their
applications. First order differential equations –
exact differential equations, integrating factors, Bernoulli equations,
existence and uniqueness theorem, applications; higher-order linear
differential equations – solutions of homogeneous and nonhomogeneous equations, method of variation of
parameters, operator method; series solutions of linear differential
equations, Legendre equation and Legendre polynomials, Bessel equation and
Bessel functions of first and second kinds; systems of first-order equations,
phase plane, critical points, stability.
Texts: 1.
G. B. Thomas (Jr.) and R. L. Finney, Calculus and Analytic Geometry, 9th
Ed., Pearson Education India, 1996. 2.
S. L. Ross, Differential Equations, 3rd Ed., Wiley India,
1984. References: 1. T.
M. Apostol, Calculus
- Vol.2, 2nd Ed., Wiley India, 2003. 2. W.
E. Boyce and R. C. DiPrima, Elementary Differential Equations and Boundary Value Problems, 9th
Ed., Wiley India, 2009. 3. E.
A. Coddington, An Introduction to Ordinary Differential Equations, Prentice Hall
India, 1995. 4. E.
L. Ince, Ordinary
Differential Equations, Dover Publications, 1958. ME
101 Engineering
Mechanics (3-1-0-8) Basic principles: Equivalent
force system; Equations of equilibrium; Free body diagram; Reaction; Static
indeterminacy. Structures: Difference between trusses, frames and beams,
Assumptions followed in the analysis of structures; 2D truss; Method of
joints; Method of section; Frame;
Simple beam; types of loading and
supports; Shear Force and bending
Moment diagram in beams; Relation among load, shear force and bending moment.
Friction: Dry friction; Description and applications of friction in wedges,
thrust bearing (disk friction), belt, screw, journal bearing (Axle friction);
Rolling resistance. Virtual work and Energy method: Virtual Displacement;
Principle of virtual work; Applications of virtual work principle to
machines; Mechanical efficiency; Work of a force/couple (springs etc.);
Potential energy and equilibrium; stability. Center of Gravity and Moment of
Inertia: First and second moment of area; Radius of gyration; Parallel axis theorem; Product of inertia, Rotation of axes
and principal moment of inertia;
Moment of inertia of simple and composite bodies. Mass moment of
inertia. Kinematics of Particles: Rectilinear motion; Curvilinear motion; Use
of Cartesian, polar and spherical coordinate system; Relative and constrained
motion; Space curvilinear motion. Kinetics of Particles: Force, mass and
acceleration; Work and energy; Impulse and momentum; Impact problems; System
of particles. Kinematics and Kinetics of Rigid Bodies: Translation; Fixed
axis rotational; General plane
motion; Coriolis acceleration; Work-energy; Power; Potential energy; Impulse-momentum and associated
conservation principles; Euler
equations of motion and its application. Texts 1. I. H. Shames, Engineering Mechanics:
Statics and Dynamics, 4th Ed., PHI, 2002. 2.
F. P. Beer and E. R. Johnston, Vector Mechanics for Engineers, Vol I - Statics, Vol
II – Dynamics, 3rd Ed., Tata McGraw Hill, 2000. References 1. J.
L. Meriam and L. G. Kraige,
Engineering Mechanics, Vol I –
Statics, Vol II – Dynamics, 5th
Ed., John Wiley, 2002. 2. R. C. Hibbler,
Engineering Mechanics, Vols. I
and II, Pearson Press, 2002. PH 102 Physics
- II
(2-1-0-6) Vector Calculus: Gradient, Divergence and
Curl, Line, Surface, and Volume integrals, Gauss's divergence theorem and
Stokes' theorem in Cartesian, Spherical polar, and
Cylindrical polar coordinates, Dirac Delta function. Electrostatics: Gauss's law and its
applications, Divergence and Curl of Electrostatic fields, Electrostatic
Potential, Boundary conditions, Work and Energy, Conductors, Capacitors,
Laplace's equation, Method of images, Boundary value problems in Cartesian
Coordinate Systems, Dielectrics, Polarization, Bound Charges, Electric
displacement, Boundary conditions in dielectrics, Energy in dielectrics,
Forces on dielectrics. Magnetostatics: Lorentz force, Biot-Savart and Ampere's laws and their applications,
Divergence and Curl of Magnetostatic fields,
Magnetic vector Potential, Force and torque on a magnetic dipole, Magnetic
materials, Magnetization, Bound currents, Boundary conditions. Electrodynamics: Ohm's law, Motional EMF,
Faraday's law, Lenz's law, Self and Mutual inductance, Energy stored in
magnetic field, Maxwell's equations, Continuity Equation, Poynting
Theorem, Wave solution of Maxwell Equations. Electromagnetic waves: Polarization, reflection
& transmission at oblique incidences. Texts:
References:
EE 102 Basic Electronics Laboratory (0-0-3-3) Experiments using diodes
and bipolar junction transistor (BJT): design and analysis of half -wave and
full-wave rectifiers, clipping circuits and Zener
regulators, BJT characteristics and BJT amplifiers; experiments using
operational amplifiers (op-amps): summing amplifier, comparator, precision
rectifier, astable and monostable
multivibrators and oscillators; experiments using
logic gates: combinational circuits such as staircase switch, majority
detector, equality detector, multiplexer and demultiplexer;
experiments using flip-flops: sequential circuits such as non-overlapping
pulse generator, ripple counter, synchronous counter, pulse counter and
numerical display.
3.
R.J. Tocci, Digital Systems, 6th Ed.,
2001. |
BT 201 Biochemistry (3-0-0-6) Basic
concept and design of metabolism; carbohydrate metabolism: glycolysis, gluconeogenesis,
citric acid cycle, pentose phosphate pathway, glycogen metabolism, oxidative phosphorylation; photosynthesis; fatty acid metabolism;
protein: synthesis, targeting and turnover; biosynthesis of amino acids and
nucleotides; Integration of metabolisms; hormones; enzymes: structure,
mechanism and reaction kinetics; introduction to information
metabolism. Texts:
1.
D. L. Nelson and M. M. Cox, Lehninger Principles of Biochemistry, 5th
Ed., Macmillan Worth, 2007. 2.
J. L. Tymoczko,
J. M. Berg and L. Stryer, Biochemistry, 5th Ed., W. H. Freeman, 2002. References:
1.
W. W. Parson, D. E. Vance and G. L. Zubay, Principles
of Biochemistry, Wm. C. Brown Publishers, 1995. 2.
K. E. Van Holde,
C. K. Mathews and K. G. Ahern, Biochemistry,
Pearson Education, 2000. 3.
R. K. Murray, D. K. Granner, P. A. Mayes and V. W. Rodwell,
Harper’s Biochemistry, McGraw
Hill, 2002. |
BT 202 Microbiology
(3-0-0-6) Microbial
cell structure and function; fundamentals of microbial taxonomy and
diversity; molecular tools in microbial taxonomy; microscopic techniques;
microbial nutrition; growth and control; microbial metabolism; mutations and
DNA repair; plasmids; transformation; conjugation; transduction; transposons; fundamentals of gene regulation;
fundamentals of microbial genomics; microbial pathogenicity
and diseases. Texts:
1. G.
Tortora, B. Funke and C.
Case, Microbiology, An
Introduction (International Edition), 8th Ed., Pearson
Education, 2003. 2. M.
Madigan, J. Martinko and J. Parker, Brock’s Biology of Microorganisms,
10th Ed., Prentice Hall, 2002. References:
1. R.
Y. Stanier, J. L. Ingraham,
M.L. Wheelis and P. R. Painter, General Microbiology, 5th
Ed., Macmillan Press, 1987. 2. L.
M. Prescott, J. P. Harley and D. A. Klein, Microbiology, 6th Ed., McGraw Hill, 2005. 3. J.
G. Black, Microbiology: Principles
& Explorations, 5th Ed., John Wiley & Sons Inc., 2002. 4. Benjamin
Lewin, Genes
VIII, Pearson Education, International Edition, 2004. |
BT 205 Biophysics
(2-1-0-6) Structure
and structural dynamics of DNA, RNA and proteins; techniques for monitoring
structure and dynamics: absorption, fluorescence, circular dichroism, light scattering; methods for separation and
characterization of molecules; size-exclusion chromatography;
electrophoresis; MALDI–TOF; ESI–quadrupole
mass spectrometry; thermodynamics and equilibria of
macromolecules in solution; biophysics of membranes; photobiological
processes; nerve impulse; muscle contraction; modelling
biological processes; protein folding and aggregation; protein function
(Myosin and Kinesin). Texts: 1. K.
E. van Holde, W. C. Johnson and P. S. Ho, Principles of Physical Biochemistry,
Prentice Hall, 1998. 2. C.
R. Cantor and P. R. Schimmel, Biophysical Chemistry (Parts
I, II and III), W. H. Freeman and Co., 1980. Reference:
1.
K. A. Dill and S. Bromberg, Molecular Driving
Forces. Statistical thermodynamics in Chemistry and Biology, Garland
Science, 2003. |
BT 208
Molecular Biology & Genetic
Engineering (3-1-0-8) Cell
organization and subcellular structure; structure
and properties of nucleic acids; organization of prokaryotic and eukaryotic
genomes; mechanisms of DNA replication; mutagenesis and processes of DNA
repair; transcription; translation; mechanisms of DNA recombination;
regulation of gene expression; eukaryotic RNA splicing and processing; cell cycle;
programmed cell death; cell transformation; genes in differentiation and
development; oncogenes. genetic engineering:
restriction modification enzymes; cloning vectors: plasmids, phages, cosmids, phagemids, yeast and
bacterial artificial chromosomal vectors; construction cDNA
and genomic libraries; screening of libraries: by DNA hybridization, immuno and protein assays; gene cloning and expression in
prokaryotes and eukaryotes; recombinant protein expression in E. coli, yeast
and baculovirus; mammalian cell expression vectors
(Selectable markers, Two-hybrid expression system); chimeric
vectors; Site-directed mutagenesis and its applications; transposons,
gene targeting; site specific recombination; polymerase chain reaction (PCR);
applications of reverse transcription PCR (RT-PCR) and real time PCR;
principles and applications of DNA finger printing; gene mapping by
restriction fragment length polymorphism (RFLP); application of differential
display and subtractive hybridization. Texts:
1. B.
Alberts, A. Johnson, J. Lewis, M. Raff, K. Roberts
and P. Walter, Molecular Biology of
Cell, 4th Ed., Garland Publishing, 2002. 2. H.
Lodish, A. Berk, S. L. Zipursky, M. P. Scott and J. Darnell, Molecular Cell Biology, 4th
Ed., W. H. Freeman & Co., 2003.
References:
1.
B. Lewin, Genes
VIII, International Edition, Pearson Education, 2004. 2.
B. R. Glick and J. J. Pasternak, Molecular
Biotechnology: Principles and Applications of Recombinant DNA, 3rd
Ed., ASM Press, 2003. 3. R.
M. Twyman, S. B. Primrose and R. W. Old, Principles of Gene Manipulation,
Blackwell Science, 2001. |
BT 290 Biomolecular Analysis Laboratory (0-0-6-6) Theory, operation and handling of instruments to be used in this
Lab course; Estimation of DNA in solution; Estimation of protein in solution;
Estimation of carbohydrate; Enzyme Linked Immuno
Assay; Purification of protein by chromatography; Equilibrium unfolding of a
protein; Gel electrophoresis of protein; Study of Enzyme kinetics. Texts: 1.
R. Boyer, Modern Experimental
Biochemistry, 3rd Ed., Pearson Education
(Singapore) Pvt. Ltd., 2001. 2. R. L. Switzer and L. F. Garrity, Experimental
Biochemistry, 3rd Ed., W. H. Freeman, 1999. Reference: 1.
K. Wilson and J. Walker (ed.), Practical
Biochemistry, Principles and Techniques, Cambridge University Press,
1995. |
BT 301 Bioinformatics and Computational Biology (2-0-0-4) Introduction
to bioinformatics; Gene bank sequence database; submitting sequences to
database; Analysis of genome content and organization; Analysis of protein
content and organization; Analysis of protein structures; Identification of
signature motifs in proteins; Secondary structure prediction; Comparative
genomics and proteomics, Basics of aligning nucleic acid and protein
sequences; Phylogenetic analysis using internet;
protein structure-function relationships; computational analysis of protein-ligand binding; enzyme catalysis and protein folding.
Texts:
1.
D. Baxevanis, and B. F. F. Ouellette, Bioinformatics – A Practical Guide to the Analysis of Genes and
Proteins, 2nd Ed., John Wiley and Sons Inc., 2001. 2. A. R. Leach, Molecular Modelling:
Principles and Applications, Addison-Wesley Pub. Co. 1997. References:
1.
P. E. Bourne and H. Weissig, Structural Bioinformatics, WILEY, 2003. 2.
T. Lengauer, Bioinformatics
- From Genomes to Drugs, Vols 1 and 2,
Wiley-VCH, 2002. |
BT 303 Immunology (3-0-0-
6) Introduction
to immune system; evolution of immunity; elements of Immune system; cell
migration & inflammation; immunogens &
antigens; antibody structure and function – catalytic Antibodies,
antigen antibody interactions, humoral and cellular
immunity – MHC, antibody diversity, dendritic
cells (APC), control mechanisms in the immune response cytokines –
complement – autoimmunity; immunity to different types of pathogens,
vaccination; tumor immunology; immune diseases and disorders; transplantation
immunology; co-stimulatory pathways; hybridoma and
immunoassays. Texts: 1. I. Roitt, J.
Brostoff and D. Male, Immunology, 6th Ed., Harcourt Publishers, 2001. 2. R. A. Goldsby,
T. J. Kindt, B. A. Osborne and J. Kuby, Immunology,
W. H. Freeman & Co, 2003. References:
1. A. K. Abbas, A. H. Lichtman and J. S.
Pober, Cellular
and Molecular Immunology, W. B. Saunders Co., 2000. 2. D. M.
Weir and J. Stewart, Immunology,
Churchill Livingstone, 1997. 3. A. Cooke, M. Owen, J. Trowsdale, B. Champion and D. K. Male, Advanced Immunology, Mosby Publ.,
1996. 4.
R. Coico, G. Sunshine and E. Benjammini, Immunology:
A short Course, Wiley-Liss Publ., 2003. |
BT 309 Plant
Biotechnology (3-0-0-6) Plant
morphogenesis; cellular totipotency; in vitro culture; protoplast isolation and culture; somatic hybridization; haploid and triploid production; somaclonal variation; embryo rescue and synthetic seeds; production of
secondary metabolites; cryopreservation and conservation of germplasm; plant gene structure, function and regulation;
plant transformation; marker genes; promoters; molecular analysis;
chloroplast transformation; genetic engineering for resistance to insects,
pests, virus, pathogens and tolerance to herbicides; gene silencing;
metabolic engineering; molecular farming; molecular markers for plant improvement; plant
genomics. Texts: 1. A. Slater, N. Scott and M. Fowler, Plant Biotechnology: The genetic
manipulation of plants, Oxford University Press, 2003.
2. S.
S. Bhojwani and M. K. Razdan,
Plant Tissue Culture: Theory and
Practice, Elsevier, 1996. . References:
1. J.
Hammond, P. McGarvey and V. Yusibov,
Plant Biotechnology: New Products and Applications,
Springer Verlag, 1999. 2. P.
Jones, P. J. Jones and J. M. Sutton, Plant
Molecular Biology: Essential Techniques, John Wiley & Sons, 1997. 3. Potrykus
and G. Spangenberg, Gene Transfer to Plants, Springer Verlag,
1995. |
BT 380 Molecular
Biotechnology Laboratory (0-0-6-6) Theory, operation and handling of instruments to be used in
this Lab course; Aseptic Techniques for plant and microbial culture; In vitro
plant regeneration by Organogenesis, Somatic embryogenesis, Meristem and Nodal segment Culture; Meiotic and Mitotic
Chromosome preparation; Isolation of pure microbial culture and
quantification of viable cells; Preparation of chemically competent E. coli
cells; Transformation of E. coli; Small scale isolation of recombinant
plasmid from E. coli; Analysis of the recombinant plasmid using Restriction Endonucleases; Agrobacterium-mediated
plant transformation & transient reporter gene expression assay; Analysis
of a transgene by PCR. Texts: 1.
H. S. Chawla, Laboratory
Manual for Plant Biotechnology, Oxford & IBH Publishing Co., New
Delhi, 2003 2. S. J. Karcher, Molecular Biology: A Project Approach,
Academic Press, 2001. References: 1.
J. Sambrook, D. W. Russell and J. Sambrook, Molecular
Cloning, A laboratory Manual, Cold Spring Harbor Laboratory, USA, 1999. 2.
J. G. Chirikjian, Biotechnology: Theory and Techniques (Genetic Engineering,
Mutagenesis and Separation Technology), Jones & Bartlett Publishers,
U.K., 1995. 3.
H. Jones and John M. Walker, Plant Gene
Transfer and Expression Protocols: Methods in Molecular Biology, 49, Humana Press, 1996. 4.
J. G. Chirikjian, Biotechnology: Theory and Techniques (Plant Biotechnology, Animal
Cell Culture and Immunobiotechnology), Jones
& Bartlett Publishers, U.K., 1996. 5. D. M. Glover and B. D Hames, DNA Cloning
II, IRL Press, 1995. |
BT 310 Bioinformatics
and Computational Biology Laboratory (0-0-6-6) Molecular databases and their organization; Analysis of
Restriction sites in a known DNA fragment; Design of a specific PCR primer
for an amplicon; Homology search algorithm; Multiple
sequence alignment and phylogenetic analysis; Gene
identification strategies; Identification of structural and functional
motifs; Visualization and analysis of protein structure; Homology Modeling of
protein. Texts: 1.
B. Bryan, Bioinformatics computing: the
complete practical guide to bioinformatics for lifescientists,
Prentice Hall,
2000. 2. S. Misener
and S. A. Krawetz, Bioinformatics: methods and protocols, Humana Press, 2000. References:
1.
D.
Baxevanis and B.F. F. Ouellette, Bioinformatics: a practical guide to the
analysis of genes and proteins, 2nd Ed., John Wiley & Sons,
2001. 2.
S. A. Krawetz and D. D. Womble, Introduction
to bioinformatics: a theoretical and practical approach, Humana Press,
2003. 3.
D. Higgins and W. Taylor (ed.), Bioinformatics:
sequence, structure and databanks-a practical Approach, Oxford, 2000. |
BT
302 Biochemical
Engineering (3-1-0-8) Unique features of the field, definitions and
concepts, introduction of organisms as mass and energy consumers, metabolic stoichiometry and energetics,
kinetics of substrate utilization, product formation and biomass production
in cell cultures, Transport phenomena in bioprocess systems: gas-liquid mass
transfer in cellular systems, determination of oxygen transfer rates, Design
and analysis of biological reactors: batch, continuous, fed-batch, fluidized
bed reactor, packed bed reactor, bubble column, trickle bed reactor, animal and plant cell reactor
technology, aeration and agitation, Instrumentation and control: physical,
chemical and biosensors, online sensors, computers and interfaces, scale up, applied enzyme catalysis;
media and air sterilization, product recovery, bioprocess economics. Texts:
1.
H. W. Blanch and D. S. Clark, Biochemical
Engineering, Marcel, Dekker Inc., 1996. 2. J.
E. Bailey and D. F. Ollis, Biochemical Engineering Fundamentals, 2nd Ed.,
McGraw-Hill Inc., 1986. References:
1. P. A. Belter, E. L. Cussler and W. S. Hu, Bioseparations: Downstream Processing for Biotechnology,
John Wiley & Sons, 1988. 2.
H. J. Rehm and G. Reed, Biotechnology-A
multi- Volume Comprehensive Treatise, Vol 3, 2nd Ed., VCH, 1993 3.
M. Moo-Young, Comprehensive
Biotechnology, Vol 2, Pergamon
Press, 2004 4.
S. Aiba, A. E. Humphrey and N. Millis, Biochemical Engineering, Prentice-Hall
1978 5. P.
F. Stanbury and A. Whitaker, Principles of fermentation technology, Pergamon
press, 1984 6.
H. C. Vogel and C. L. Tadaro, Fermentation
and Biochemical Engineering Handbook - Principles, Process Design, and
Equipment, 2nd Ed., William Andrew Publishing/Noyes, 1997. |
BT 305 Industrial Microbiology (3-0-0-6) Pre-requisite: BT 202 or equivalent. Basis
and development of Industrial Fermentation Processes (Screening cultures, media,
inoculum, scale-up); Control of microorganisms; Fermentation Processes; Microbial
production of Antibiotics, Alcohols, Enzymes, Organic acids, Amino acids,
Vitamins, Biopolymers; Microbial
polysaccharides; Bioplastics; Biosurfactants; Bioinsecticides; Pigments and flavors with their applications; Microbial
leaching; Bio-transformation;
Bio-degradation; Food
production involving microorganisms and their products; Fermentation
involving genetically engineered microbes; Industrial applications of extremophiles; Safety aspects of industrial
processes. Texts: 1. M.
A. Malden, Industrial Microbiology: An
Introduction, Blackwell Science, 2001. 2.
A. N. Glazer and H. Nikaido, Microbial Biotechnology: Fundamentals of Applied Microbiology, W.
H. Freeman & Co, 1995. References: 1. L.
Demain, R. M. Atlas, G. Cohen, C. L. Hershberger,
W. S. Hu, D. H. Sherman, R. C. Wilson and J. H.
David, Manual of Industrial
Microbiology and Biotechnology, 2nd Ed, ASM Press, 1999. 2.
H. J. Rehm and G. Reed, Biotechnology: A Comprehensive Treatise, VCH publisher, 2001. |
BT 306 IPR,
Ethics and Bio-Safety (3-0-0-6) General overview of
Intellectual Properties; Patent and
utility models; Design and trademark; Trade secret and unfair competition; New
plant varieties and geographical indication; Copyright and related rights; International
intellectual property treaties; Patents structure and classification; Patenting
procedures; Economic impact of the patent system and legal issues; Licensing and enforcing intellectual
property; Commercializing an invention; Case studies; Scope of intellectual
properties in India –
Biodiversity and Traditional knowledge; Biosafety- classification and
description of biosafety levels; Design of clean
rooms and biosafety labs; Biosafety
regulations to protect nature; Growers and consumers interest and nation
interest; Potential risk from genetically modified organisms; Ethical issues
in research and case studies. Texts: 1. C.B. Raju, Intellectual Property Rights, Serial Publication, New Delhi, 2006 2. B.A.Brody and H.T. Engelhardt (Jr.), Bioethics: Reading & Cases, Person
Education, Inc., 2007 References: 1. P.N. Cheremisinoff, R. P.
Ouellette and R.M. Bartholomew, Biotechnology Applications and Research,
Technomic Publishing Co., Inc., USA, 1985. 2. D. Balasubramaniam, C.F.A. Bryce,
K. Dharmalingam, J. Green and K. Jayaraman, Concepts in Biotechnology, University
Press (Orient Longman Ltd.), 2002. 3. D. Bourgagaize, T.R. Jewell and
R. G. Buiser, Biotechnology: Demystifying the Concepts,
Wesley Longman, USA, 2000. |
BT
308 Animal
Cell Biotechnology ( 3-0-0-6) Pre-requisite: BT 208 or equivalent Animal
cell biotechnology: Animal cell and tissue engineering; Animal cell culture techniques
relevant to mRNA knockdown (e.g. antisense and ribozyme
technology); generation of immortalized cell lines. In vitro organogenesis;
Stem Cells; Differentiation of animal and human cells; Animal cloning;
Mechanisms of drug resistance and cell death; Basic developmental Biology;
Structure and organization of tissues; Cell Surface markers; FACS analysis;
Cell Migration: control of cell migration in tissue engineering;
Transplantation immunology. Texts:
1.
T. A. Brown, Gene Cloning and DNA
Analysis: An Introduction, Blackwell Science, 2001. 2.
T. A. Brown, Genomes, 2nd
Ed., BIOS Scientific Publishers, 2002. References: 1.
B. R. Glick and J. J. Pasternak, Molecular
Biotechnology: Principles and Applications of Recombinant DNA, 3rd
Ed., ASM Press, 2003. 2.
R. I. Freshney, Animal
Cell Culture: A Practical
Approach, 2nd Ed., IRL Press, 1992. 3.
R. E. Spier, Encyclopedia
of Cell Technology, Vols 1 and 2, Wiley
Biotechnology Encyclopedia, John Wiley & Sons, 2000. 4.
A. Doyle, J. B. Griffiths and D. G. Newell, Cell and Tissue Culture Laboratory Procedures, John Wiley &
Sons, 1998. |
BT 330 Biochemical
Engineering Laboratory (0-0-6-6) Theory, operation and handling of instruments to be used in this
Lab course; Determination of biomass concentration by dry weight method;
Determination of bacterial specific growth rate and substrate utilization
rate in a batch operated reactor; Study of substrate inhibition kinetics of
bacterial growth in shake flask culture; Production and purification of an
enzyme from a microbial source; Determination of deactivation kinetics of a
soluble enzyme; Determination of Kla. Texts: 1.
J. E. Bailey and D. F. Ollis, Biochemical Engineering Fundamentals, 2nd Ed., McGraw-Hill
Inc., 1986. 2. P. A. Belter, E. L. Cussler and W.
S. Hu., Bioseparations:
Downstream Processing for Biotechnology, John Wiley & Sons, 1988. References: 1.
H. J. Rehm and G. Reed., Biotechnology - A Multi-volume Comprehensive Treatise, Vol.3, 2nd
Ed., VCH, 1993. 2. M. Moo-Young, Comprehensive Biotechnology, Vol 2, Pergamon Press, 2004. 3. P. F. Stanbury
and A. Whitaker, Principles of
fermentation technology, Pergamon Press, 1984. 4.
S. Aiba, A. E. Humphrey and N. Millis, Biochemical Engineering, Prentice-Hall
1978. |
BT 404 Bioseparation Engineering (3-0-0-6) Downstream
processing of products: filtration, centrifugation, sedimentation, solvent
extraction, aqueous two phase system, sorption, precipitation, chromatography
; whole broth processing; Cell separation: disruption by presses,
homogenizers, milling, sonication, and non
mechanical methods; preparative electrophoresis; product recovery schemes for
antibiotics, commercial enzymes and organic acids. Texts:
1.
T. Nagamune, S. Katoh and
T. Yonemoto, Bioseparation Engineering, Elsevier Science
Publication, 2002. 2. M.
R. Ladisch, Bioseparations
Engineering: Principles, Practice and Economics, Wiley-Inter Science,
2001. References: 1. R. G. Harrison, P. Todd,
S. Rudge and D. P. Petrides,
Biosperations Sceince and
Engineering, Oxford University Press, 2003. 2. P. A. Belter, E. L. Cussler and W. S. Hu, Bioseparations: Downstream Processing for Biotechnology,
John Wiley & Sons, 1988. |
BT 405 Environmental Biotechnology (3-0-0-6) Introduction
to environment; pollution and its control; pollution indicators; waste
management: domestic, industrial, solid and hazardous wastes; strain
improvement; biodiversity and its conservation; microbes for bioremediation
technology such as petroleum, hydrocarbon decontamination, radionuclei, toxic metal, dyes and lignin removal and xenobiotics; phytoremediation;
biomass for removal and biosorption of heavy metal
and other inorganic ions; removal of volatile organic compounds from waste
gas. Texts:
1. B.
Ritmann and P. L. McCarty, Environmental Biotechnology: Principle & Applications, 2nd
Ed., McGraw Hill Science, 2000. 2. G.
M. Evans and J. C. Furlong, Environmental
Biotechnology: Theory and Applications, Wiley Publishers, 2002. References: 1. H. S. Peavy, D. R. Rowe
and G. Tchobanoglous, Environmental Engineering, McGraw-Hill Inc., 1985. 2. J. S. Devinny, M. A. Deshusses and T. S. Webster, Biofiltration for Air Pollution Control, CRC Press,
1998. 3. H. J. Rehm and G. Reed, Biotechnology – A Multi-volume
Comprehensive Treatise, Vol. 11,
2nd Ed., VCH Publishers Inc., 1993. |
BT 401 Frontiers
in Biotechnology (3-0-0-6) Introduction to
microfluidics and microfabrication,
micropatterning and lithographic techniques,
applications of microfluidic systems in biology;
Biosensors and biofuel cells; Mechanisms,
therapeutic and computational aspects of cancer, AIDS and neurodegenerative
disorders; Systems biology; Modern analytical techniques in biotechnology. Texts: 1. J.Cooper, and
T. Cass, Biosensors, 2nd Ed., Oxford University
Press, 2004. 2. L. Pecorino, Molecular Biology of Cancer: Mechanisms, Targets, and Therapeutics,
2nd Edn., OUP, 2008. References: 1. J.B. Park and J.D. Bronzino,
Biomaterials: Principles and Applications. CRC Press. 2002. 2. T.S. Hin
(ed.), Engineering Materials for Biomedical Applications. World
Scientific. 2004. 3. V. C. Yang and T. T. Ngo (eds.), Biosensors and their applications, Kluwer Academic/Plenum Publishers, Moscow, 2000. 4. T. Finkel and
J. S. Gutkind (eds.), Signal transduction and human disease, Wiley Interscience,
2003. |
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