Department
              of
          Biosciences and Bioengineering
Indian Institute of Technology Guwahati

Faculty:

 

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Department of Biosciences & Bioengineering
Indian Institute of Technology Guwahati
Guwahati 781039

Telephone:
(0361) 258-2250
Fax: (0361) 258-2249
E-mail: biooff@iitg.ernet.in

  

Dr. Siddhartha Sankar Ghosh, Ph.D.
Professor

Contact

Dr. Siddhartha Sankar Ghosh
Professor
Department of Biotechnology
Indian institute of Technology Guwahati
Tel: 0361-258-2206
Fax: 0361-258-2249
Email: sghosh@iitg.ernet.in

Educational Qualifications


- Ph. D. (Mol. Biology): 1998, Indian Institute of Chemical Biology (IICB), Kolkata
- M. Sc. (Biochemistry): 1991, University College of Science, Kolkata

- B. Sc. (Chemistry Honors): 1988, Ramakrishna Vivekananda Mission College, Kolkata

 
Professional Experience

- Professor:  July 2012 onwards, IITG
- Associate Professor: July 2007- June 2012, IITG
- Assistant Professor: March 2003-July 2007, IITG

- Post Doctoral Fellow: March 1998-February 2003, Albert Einstein College of Medicine, NY, USA
 
Administrative Experience

- Head, Centre for Nanotechnology: April 2009- June 2013, IITG

Member of Institute committee:
- Institute Biosafety Committee (Continuing)

- Institute Animal Ethics Committee (Continuing)

 
Research Areas

Cancer Gene Therapy
Nanobiotechnology
Molecular Pathways Involving Drug Resistance
 

A. Cancer Gene Therapy:
Our group focuses on development of new generation gene therapy vectors. This mainly includes development of suicide gene therapy for cancer. The efficacy and the molecular mechanism associated with suicide gene therapy are the primary objectives of our investigations. Rational Designing of suicide proteins with improved specificity towards the prodrug for gene therapy application is another major thrust. We have also been able to demonstrate apoptotic signaling pathways involving suicide gene therapy. The detail molecular investigation would help us to choice the right vector for cancer gene therapy

Schematic of Suicide Gene Therapy

B. Nanobiotechnology:
We have set up infrastructure facilities for interdisciplinary collaborative research in the field of nanoscience and nanotechnology supported by extramural funding at the Centre for Nanotechnology. The major area is to develop new nanoparticles, nanocomposites and nanocarriers and evaluate their antimicrobial and anticancer activities. We are perusing research to understand molecular mechanisms of nanoparticle mediated cell cytotoxicity. Other areas, such as, bioimaging using C-dots, metal nanoclusters, gene delivery using quantum dot embedded nanocarriers are also being persuaded.



Schematic representation of silver nanoparticles induced apoptosis

Ref: Colloids and Surfaces B: Biointerfaces (2010), 77 240–245

C. Molecular Pathways Involving Drug Resistance:
The NFkB overexpression triggers drug resistance in many types of cancer by up-regulating anti-apoptotic genes. IkBa, an endogenous protein and natural inhibitor of NFkB, blocks translocation of NFkB from cytoplasm to nucleus and thereby, sensitizes cancer cells to external stimuli. We have demonstrated therapeutic efficacy of recombinant IkBa loaded hydrogel nanocarrier on cancer cells. Furthermore, we are at present pursuing research on cloning, purification and molecular effects of various cytokines and transcription factors involved in cell signaling for drug resistant phenotype. Combination therapy using drugs and genes and the role of induced cancer stem cells in drug resistant cancer are also under investigation.

Schematic representation of recombinant IkBa mediated cancer therapy

Ref: RSC Advances, (2013), 3 (33), 14123 - 14131

Teaching

-Various undergraduate, post graduate and PhD level courses at the department, which include fundamental, advance and applied courses in the filed of molecular biology, gene therapy and related areas.

- Interdisciplinary courses at the Centre for Nanotechnology for the students of other disciplines, such as Nanobiosciences (NT 710) is an open elective course.

Sponsored Research Projects

Ongoing projects: 

  1. Project Coordinator, DBT Program support on “Fundamental Molecular Investigations in Biotechnology Phase -II” (2016- 2021).

Implemented at the Department of Biosciences and Bioengineering, IITG  

Overview of DBT Program Support: After successful completion of Phase –I of the DBT program support project, the Phase-II of this interdisciplinary research program involving five faculty members of the Department of Biosciences and Bioengineering, IITG has been approved in 2016. In Phase-I a state-of the art research facility (DBT Program Support Facility), was set up to pursue research on four challenging topics with potential application in healthcare and agriculture sector. The major leads obtained in Phase–I program will be investigated in details in the Phase II. The phase-II program consist of a core grant to support major equipment facilities and four different R&D projects namely, a) combination therapy using suicide genes and recombinant antibody b) molecular interaction of nanomaterials c) biocatalysis and bioelectronics d) plant functional genomics.  Phase-II comprises a long term strategy to investigate specific objectives in above areas using modern biotechnological tools and provide viable knowledge based solutions. Human resource development is the major area of the program. Many PhD students worked in phase-I of this program have joined as faculty in IITs, NITs and Central Universities. Laboratory oriented short-term training courses are being routinely organized to train researchers and young scientist across different fields. 

  1. Principle Investigator, DBT supported project entitled “Novel nanocluster based targeted anticancer theranostics” (2016- 2019).

Implemented at the Centre for Nanotechnology, IITG

Over View of the Project: This R&D project includes other investigators from Chemistry and Biotechnology. The project aims at the development of composite biofriendly nanomaterials for targeted delivery to cancer cells. The nanomaterials mostly comprise of metals and polymers. Our detail investigations on molecular pathways would help to establish newer nanomaterials for therapeutic applications. 

  1. Co-Principle Investigator, DEITY supported project entitled “Centre for Excellence in Research and Development of Nanoelectronic Theranostic Devices” (2014-2019)

Implemented at the Centre for Nanotechnology, IITG

Over View of the Project: This R&D project includes other investigators from various departments. The Centre for Excellence In Nanoelectronics & Theranostic Devices is established with a vision to develop devices, state-of-art fabrication and characterization facilities at the Centre for Nanotechnology, IIT Guwahati, for the development of society, which will be the first of its kind in the NE region. 

Completed Projects: 

  1. Project Coordinator, DBT Program support on “Fundamental Molecular Investigations in Biotechnology Phase -I” (2008- 2014).

  2. Principle Investigator, DBT supported project entitled “Novel Nanoscale Materials Targeted towards Antimicrobial and Anticancer Activities” (2010- 2014).

  3. Principle Investigator, MHRD funded project entitled “Construction of Prodrug Gene Expression Systems” (2004-2006). Implemented at the Department of Biotechnology

  4. Principle Investigator, CSIR funded project entitled “Sleeping Beauty (SB) Transposon Mediated Sequence Specific Delivery and Activation of Prodrug Gene in Hepatocellular Carcinoma Cells” (2006-2009). Implemented at the Department of Biotechnology

  5. Principle Investigator, DBT funded project entitled “Nanoscale Materials with Therapeutic Implications” (2007-2011). Implemented at the Centre for Nanotechnology

  6. Co- Investigator, DBT funded project entitled, “ Inhibitor Based Selection of Blocking Antibodies against Heparin-binding EGF-like Growth Factor: Developing Potent Molecules for Antibody-based Cancer Therapy” (2007-2010), Implemented at the Department of Biotechnology

  7. Co- Investigator, DRDO funded project entitled, “Development of Nanoscale Materials for Bacteria Removal from Surface Water” (2010-2012). Implemented at the Centre for Nanotechnology.

  8. Associate Faculty Investigator, DST funded project entitled “Engineering nanoscale materials and their applications in nanotechnology” (2005-2010). Implemented at the Centre for Nanotechnology.

  9. Associate Faculty Investigator, DST funded project entitled “Novel Nanoscale Materials: Generation, Characterization, and Device Applications” (2005-2010). Implemented at the Centre for Nanotechnology.

     Journal Publications:

  1. Raza A, Ghoshal A, Chockalingam S, Ghosh SS(2017), Connexin-43 enhances tumor suppressing activity of artesunate via gap junction-dependent as well as independent pathways in human breast cancer cells, Scientific Reports,DOI: 10.1038/s41598-017-08058-y[Nature Publishing Group]

  2. Narayanan S, D Dutta, N Arora, Sahoo L, Ghosh SS (2017),  Phytaspase-loaded, Mn-doped ZnS quantum dots when embedded into chitosan nanoparticles leads to improved chemotherapy of HeLa cells using in cisplatin, Biotechnology Letters,DOI: 10.1007/s10529-017-2395-1 [Springer publishing group]

  3. Vanitha S, Bidkar AP, Shome R, Banerjee A, Chaubey N, Ghosh SS,Sanpui P (2017), Studying in vitro phagocytosis of apoptotic cancer cells by recombinant GMCSF-treated RAW 264.7 macrophages, International Journal of Biological Macromolecules, 102:1138-1145 [Elsevier publishing group].

  4. Barman U, Mukhopadhyay G, Goswami N, Ghosh SS, Paily  P. Roy (2017), Detection of Glutathione by Glutathione-S-Transferase- Nanoconjugate Ensemble Electrochemical Device, IEEE Transactions on NanoBioscience,DOI: 10.1109/TNB.2017.2698241[IEEE publishing group].

  5. Bhuyan T, Singh AK, Dutta D, Unal A, Ghosh SS, Bandyopadhyay D (2017), Magnetic Field Guided Chemotaxis of iMushbots for Targeted Anticancer Therapeutics,ACS Biomaterials Science &Engineering, DOI:10.1021/acsbiomaterials.7b00086[ACS publishing group]

  6. Narayanan S, Sanpui P, Sahoo L, Ghosh SS (2017), Heterologous expression and functional characterization of phytaspase, a caspase-like plant protease, International Journal of Biological Macromolecules, 95,288-293 [Elsevier publishing group].

  7. Narayanan S, Sanpui P, Sahoo L, Ghosh SS (2017), Tobacco phytaspase: Successful expression in a heterologous system,   Bioengineered, DOI:10.1080/21655979.2017.1292187[Taylor & Francis Publishing Group]

  8. Dutta D, Chattopadhyay A, Ghosh SS (2016),Cationic BSA Templated Au-Ag Bimetallic Nanoclusters As a Theranostic Gene Delivery Vector for HeLa Cancer Cells, ACS Biomaterials Science & Engineering, 2(11),2090-2098[ACS publishing group]

  9. Chatterjee B, Sahoo AK, Chattopadhyay A, Ghosh SS (2016),Chattopadhyay Interactive luminescent gold nanocluster embedded dsDNA and cisplatin as model nanoparticles for cancer theranostics,RSC Advances, 6,113053-113057 [RSC publishing group]

  10. Arora N, Ghosh SS (2016), Functional Characterizations of Interactive Recombinant PTEN-Silica Nanoparticles for Potential Biomedical Applications,RSC Advances,6, 114944-114954 [RSC publishing group]

  11. Ghoshal A, Goswami U, Raza A, Chattopadhyay A, Ghosh SS (2016), Recombinant sFRP4 bound Chitosan-Alginate Composite Nanoparticles Embedded with Silver Nanoclusters for Wnt/b-catenin targeting in Cancer TheranosticsRSC Advances, 6, 85763-85772[RSC publishing group]

  12. Sahoo A, Goswami U, Dutta D, Banerjee S, Chattopadhyay A, Ghosh SS (2016), Silver nanoclusters embedded composite nanoparticles for targeted prodrug delivery in cancer theranostic, ACS Biomaterials Science & Engineering, 2 (8), 1395-1402 [ACS publishing group]

  13. Vanitha S.,Goswami U, Chaubey N, Ghosh SS and Sanpui P(2016),Recombinant Human Granulocyte Macrophage Colony Stimulating Factor (hGM-CSF): Possibility of Nanoparticle-Mediated Delivery in Cancer Immunotherapy, Bioengineered, DOI: 10.1080/21655979.2016.1212136 [Taylor & Francis Publishing Group].

  14. Ghoshal A and Ghosh SS (2016), Antagonizing Canonical Wnt Signaling Pathway by Recombinant Human sFRP4 Purified from E. coli and its Implications in Cancer Therapy, Molecular and Cellular Biochemistry, 418 (1-2), 119-135. [Springer publishing group].

  15. Narayanan S, Sanpui P, Sahoo L, Ghosh SS (2016),Unravelling the potential of a new uracil phosphoribosyltransferase (UPRT) from Arabidopsis thaliana in sensitizing HeLa cells towards 5-fluorouracil, International Journal of Biological Macromolecules, 91:310-316[Elsevier publishing group].

  16. Dutta D, Sahoo AK, Chattopadhyay A, Ghosh SS (2016), Bimetallic silver nanoparticle-gold nanocluster embedded composite nanoparticles for cancer theranostics, Journal of Materials Chemistry, 4, 793-800[RSC publishing group]

  17. Vanitha S.,Goswami U, Chaubey N, Ghosh SS and Sanpui P(2016), FunctionalCharacterization of Recombinant Human Granulocyte Colony StimulatingFactor (hGMCSF) Immobilized onto Silica Nanoparticles, Biotechnology Letters, 38(2), 243-249 [Springer publishing group]

  18. Ghoshal A, Goswami U, Sahoo AK, Chattopadhyay A, Ghosh SS (2015), Targeting Wnt Canonical Signaling by Recombinant sFRP1 Bound Luminescent Au-Nanocluster Embedded Nanoparticles in Cancer Theranostics, ACS Biomaterials Science & Engineering, 1 (12), 1256–1266 [ACS publishing group]

  19. Marvi DK, Sahoo AK, Goswami U, Prasad DS, Sahoo L, Ghosh SS(2015), Phytogenic “green synthesis” of silver nanoparticles with enhanced antibacterial and anticancer activity,International J Pharm Bio Sci., 6(4): 482 – 493 [IJPBS publishing group]

  20. Md Asif Raza, V. Kohila, Ghosh SS (2015), Redesigned E.coli cytosine deaminase: a new facet of suicide gene therapy, The Journal of Gene Medicine  7(6-7):132-9 [Wiley publishing group]

  21. Md Palashuddin Sk, Goswami U,Ghosh SS and Chattopadhyay A (2015), Cu(2+)- embeddedCarbon Nanoparticle as an Anticancer Agent, Journal of Materials Chemistry B, 3, 5673--5677 [RSC publishing group]

  22. Khandelia R, Bhandari S, Pan UN, Ghosh SS andChattopadhyay A (2015),Gold Nanocluster Embedded Albumin Nanoparticles for Two-Photon Imaging of Cancer Cells Accompanying Drug Delivery, Small,11(33): 4075-81 [Wiley publishing group]

  23. GhoshalA and Ghosh SS (2015), Expression, Purification, and Therapeutic Implications of Recombinant sFRP1, Applied Biochemistry and Biotechnology,175, 4, 2087-2103. [Springer publishing group]

  24. ChaubeyN and Ghosh SS (2015),Overexpression of Granulocyte Macrophage Colony Stimulating Factor in Breast Cancer Cells Leads Towards Drug Sensitization, Applied Biochemistry and Biotechnology,175,4, 1948-1959 [Springer publishing group]

  25. Ghosh R, Goswami U, Ghosh SS, Paul A and Chattopadhyay(2015), Synergistic Anticancer Activity of Fluorescent Copper Nanoclusters and Cisplatin Delivered through a Hydrogel Nanocarrier, ACS Applied Materials and Interfaces, 1, 7(1), 209-222 [ACS publishing group]

  26. Mallick S, Sanpui P, Ghosh SS, Chattopadhyay A, Paul A(2015), Synthesis, characterization and enhanced bactericidal action of a chitosan supported core–shell copper–silver nanoparticle composite, RSC Advances, 5, 12268-12276 [RSC publishing group]

  27. Thakur R, Chattopadhyay D, Ghosh SS, Mukherjee AK (2015), Elucidation of procoagulant mechanism and pathophysiological significance of a new prothrombin activating metalloprotease purified from Daboiarusseliirusseliivenom. Toxicon100, 1-12

  28. Banerjee S, Sahoo AK, Chattopadhyay A, Ghosh SS (2014), Chemosensitization of IκBα overexpressing glioblastoma towards anti-cancer agents, RSC Advances,4, 39257-39267 [RSC publishing group]

  29. Chockalingam S and Ghosh SS (2014), Macrophage Colony Stimulating Factor and Cancer, Tumor biology,35(11),10635-44[Springer publishing group]

  30. Banerjee S, Sahoo AK, Chattopadhyay A, Ghosh SS (2014), Recombinant IκBα-loaded curcumin nanoparticles for improved cancer therapeutics, Nanotechnology, 25 (2014) 345102 (11pp), doi:10.1088/0957-4484/25/34/345102 [IOP publishing group]

  31. Sailapu S K, Sahoo AK, Ghosh SS and Chattopadhyay A (2014), Hierarchical Logic Structures Based on Responsive Fluorescent Gold Nanoclusters, Small,29; 10(20), 4067-71 [Wiley publishing group]

  32. Khandelia R, Jaiswal A, Ghosh SS and Chattopadhyay A (2014), Polymer coated Gold Nanoparticle-Protein Agglomerates as Nanocarriers for Hydrophobic Drug Delivery, J. Mater. Chem. B, 2 (38), 6472 – 6477 [RSC publishing group]

  33. Chaubey N, Sahoo A, Chattopadhyay A and Ghosh SS (2014), Silver nanoparticle loaded PLGA composite nanoparticles for improving therapeutic efficacy of recombinant IFNγ by targeting the cell surface, Biomaterials Science, 2, 1080-1089 [RSC publishing group]

  34. Ghosh R, Sahoo A, Ghosh SS, Paul A and Chattopadhyay A (2014), Blue Emitting Copper Nanoclusters Synthesized in the Presence of Lysozyme as Candidates for Cell Labeling, ACS Applied Materials & Interfaces, 6(6):3822-8.[ACS publishing group]

  35. Sharma S, Chockalingam S, Sanpui P, Chattopadhyay A and Ghosh SS (2014), Silver Nanoparticles impregnated Alginate-ChitosanblendedNanocarrier Induces Apoptosis in Human Glioblastoma Cells, Advanced Healthcare Materials, 3, 106–114.[Wiley publishing group]

  36. Majumdar S, Sarmah B, Gogoi D, Banerjee S, Ghosh SS, Chattopadhyay P, AK Mukherjee AK (2014). Characterization, mechanism of anticoagulant action, and assessment of therapeutic potential of a fibrinolytic serine protease (Brevithrombolase) purified from Brevibacillus brevis strain FF02B, Biochimie, 103:50-60[Elsevier publishing group]

  37. Chakraborty M, Ghosh SS andGoswami P (2014), Molecular Characterization and Expression of a Novel Alcohol Oxidase from Aspergillusterreus MTCC6324, PLoS ONE, 9(4):e95368. doi: 10.1371/journal.pone.0095368 [Public Library of science publishing group]

  38. Yata V, Banerjee S, Ghosh SS (2014), Folic acid conjugated-bio Polymeric nanocarriers: synthesis, characterization and In vitro delivery of prodrug converting enzyme, Advanced Science, Engineering and Medicine, (6) 4, 388-392(5)[American Scientific  publishing group]

  39. Begum R, Sahoo A, Ghosh S.S and Chattopadhyay A (2014), Recovering Hidden Quanta of Cu2+-doped ZnS Quantum Dots in Reductive Environment, Nanoscale, 6, 953-96, [RSC publishing group]

  40. Sahoo A, Banerjee S, Ghosh SS and Chattopadhyay A (2014), Simultaneous RGB emitting Au nanoclusters in chitosan nanoparticles for anticancer gene theranostic, ACS Applied Materials & Interfaces, 6 (1), 712–724. [ACS publishing group]

  41. Jaiswal A, Gautam PK, Ghosh SS and Chattopadhyay A (2014), Carbon dots mediated room-temperature synthesis of gold nanoparticles in poly (ethylene glycol), Journal of Nanoparticle Research, 16 (1), 1-14. [Springer publishing group]

  42. Chockalingam S and Ghosh SS (2013), Amelioration of cancer stem cells in Macrophage Colony Stimulating Factor-Expressing U87MG-human glioblastoma upon 5-fluorouracil therapy, PLoS ONE,8 (12), e83877. doi:10.1371/journal.pone.0083877[Public Library of science publishing group]

  43. Banerjee S, Sahoo A, Chattopadhyay A and Ghosh SS(2013). Hydrogel nanocarrier encapsulated recombinant IκBα as a novel anticancer protein therapeutics, RSC Advances, 3 (33), 14123 - 14131 [RSC publishing group]

  44. Khandelia R, Jaiswal A, Ghosh S.S, and Chattopadhyay A (2013).Gold nanoparticle–protein agglomerates as versatilenanocarriers for drug delivery, Small,9(20): 3494-505. [Wiley publishing group]

  45. Chaubey N and Ghosh SS (2013). Molecular Cloning, Purification and Functional 4Implications of Recombinant GST Tagged hGMCSF Cytokine, Applied Biochemistry Biotechnology, 169(5):1713-26 [Springer publishing group]

  46. V. Kohila, Jaiswal A, Ghosh SS (2012). Rationally designed Escherichia coli cytosine deaminase mutants with improved specificity towards the prodrug 5-fluorocytosine for potential gene therapy applications, Med. Chem. Commun.,3, 1316-1322[RSC publishing group]

  47. Jaiswal A, Ghosh SS and Chattopadhyay A (2012). A quantum dot impregnated-chitosan film for heavy metal ion sensing and removal, Langmuir, 28 (44), 15687–15696. [ACS publishing group]

  48. Saikia D, BordoloiNK,Chattopadhyay P, Chockalingam S, Ghosh SS, Mukherjee AK (2012). Differential mode of attack on membrane phospholipids by an acidic phospholipase A2 (RVVA-PLA2-I) from Daboiarusselli venom, BiochimBiophysActa. 18(12):3149-57, [Elsevier publishing group]

  49. Sharma S, Sanpui P, Chattopadhyay A and Ghosh SS (2012), Fabrication of Antibacterial Silver Nanoparticle – Sodium Alginate – Chitosan Composite Films, RSC Advances, 2, 5837–5843 1322[RSC publishing group]

  50. MdPalashuddinSk, Jaiswal A, Paul A, Ghosh SS, Chattopadhyay A, Paul A (2012), Presence of Amorphous Carbon Nanoparticles in Food Caramels. Scientific Reports, doi:10.1038/srep00383 [Nature publishing group]

  51. Yata VK, Gopinath P and Ghosh SS (2012), Emerging Implications of Nonmammalian Cytosine Deaminases on Cancer Therapeutics, Applied Biochemistry Biotechnology,167(7):2103-16 [Springer publishing group]

  52. MallickS,Sharma S, Banerjee M, Ghosh SS, Chattopadhyay A, Paul A (2012), Iodine - Stabilized Cu Nanoparticle Chitosan Composite for Antibacterial applications, ACS Applied Materials & Interfaces, 4(3):1313-23 [ACS publishing group]

  53. Yata VK and Ghosh SS (2012), Investigating structure and fluorescence properties of green fluorescent protein released from chitosan nanoparticles, Materials Letters, 73, 209–211[Elsevier publishing group]

  54. Sahoo A.K, Sharma S, Chattopadhyay A and Ghosh SS (2012), Quick and simple estimation of bacteria using a fluorescent paracetamol dimer-Au nanoparticle composite, Nanoscale, 4 (5), 1688 – 1694 [RSC publishing group]

  55. Jaiswal A, Ghosh SS and Chattopadhyay A (2012), One step synthesis of C-dots by microwave mediated caramelization of poly(ethylene glycol). Chemical Communications, 48(3):407-409 [RSC publishing group]

  56. Jaiswal A, Chattopadhyay A and Ghosh SS (2012), Functional chitosan nanocarriers for potential applications in gene therapy, Materials Letters, 68(1):261-264 [Elsevier publishing group]

  57. Banerjee M, Sharma S, Chattopadhyay A and Ghosh SS (2011). Enhanced antibacterial activity of bimetallic gold-silver core-shell nanoparticles at low silver concentration.Nanoscale, 3(12):5120-5125 [RSC publishing group]

  58. Sahoo A.K,MdPalashuddinSk, Ghosh SS and Chattopadhyay A (2011). Plasmid DNA linearization in the antibacterial Action of a new Fluorescent Ag Nanoparticle-Paracetametol Dimer composite, Nanoscale, 3(10):4226-33 [RSC publishing group]

  59. Sanpui P, Chattopadhyay A and Ghosh SS (2011).Induction of apoptosis in cancer cells at low silver nanoparticle concentrations using chitosan nanocarrier, ACS Applied Materials & Interfaces, 3(2), 218-228 [ACS publishing group]

  60. YataV.K.andGhosh SS (2011). Synthesis and characterization of a novel chitosan based E. coli cytosine deaminase nanocomposite for potential application in prodrug enzyme therapy, Biotechnology Letters, 33(1), 153-157 [Springer publishing group]

  61. Yata V.K, Sen K., Kumar M.V.S. and Ghosh SS (2011). Interaction studies of E. coli uracil phosphoribosyltransferase with 5-fluorouracil for potent anticancer activity, Medicinal Chemistry Research, DOI:10.1007/s00044-011-9627-z[Springer publishing group]

  62. Jaiswal A, Sanpui P, Chattopadhyay A and Ghosh SS (2010). Investigating fluorescence quenching of ZnS quantum dots by silver nanoparticles, Plasmonics, (6), 125–132 [Springer publishing group]

  63. Das S, Sahoo A.K, Ghosh SS and Chattopadhyay A (2010).Plasmonic signatures in the composite crystals of gold nanoparticles and p-Hydroxyacetanilide (Paracetamol), Langmuir, 26(20), 15714–15717 [ACS publishing group]

  64. Sanpui P, Pandey S.B, Chattopadhyay A and Ghosh SS (2010). Incorporation of gene therapy vector in Chitosan stabilized Mn2+-doped ZnS Quantum, Material Letters, 64 (22),2534-2537 [Elsevier publishing group]

  65. P. Gopinath, Gogoi S. K, Sanpui, P, Paul, A, Chattopadhyay A, Ghosh SS (2010). Signaling gene cascade in silver nanoparticle induced apoptosis. Colloids Surface B Biointerfaces. 77(2):240-5 [Elsevier publishing group]

  66. Banerjee M, Mallick S, Paul A, Chattopadhyay A, Ghosh SS (2010). Heightened reactive oxygen species generation in the antimicrobial activity of a three component iodinated chitosan-silver nanoparticle composite. Langmuir. 20; 26(8): 5901-8 [ACS publishing group]

  67. P. Gopinath and Ghosh SS (2009). Understanding apoptotic signaling pathways in cytosine deaminase-uracil phosphoribosyl transferase-mediated suicide gene therapy in vitro. Molecular and Cellular Biochemistry. 324(1-2):21-9 [Springer publishing group]

  68. Sanpui P, Pandey SB, Ghosh SS, Chattopadhyay A. (2008). Green fluorescent protein for in situ synthesis of highly uniform Au nanoparticles and monitoring protein denaturation. Journal of Colloid Interface Science. 326(1):129-37 [Elsevier publishing group]

  69. Sanpui P, Murugadoss A, Prasad, P.V, Ghosh SS, Chattopadhyay A. (2008). The antibacterial properties of a novel chitosan-Ag-nanoparticle composite. International Journal of Food Microbiology. 24(2):142-6 [Elsevier publishing group]

  70. P. Gopinath, Gogoi S. K, Chattopadhyay A and Ghosh SS (2008). Implications of silver nanoparticle induced cell apoptosis for in vitro gene therapy. Nanotechnology. 19(7):075104. doi: 10.1088/0957-4484/19/7/075104[IOP Science publishing group]

  71. P. Gopinath and Ghosh SS (2008). Implication of functional activity for determining therapeutic efficacy of suicide genes in vitro. Biotechnology Letters. 30 (11):1913-21 [Springer publishing group]

  72. P. Gopinath and Ghosh SS (2008). Apoptotic induction with bifunctionalE.coli cytosine deaminase-uracil phosphoribosyltransferase mediated suicide gene therapy is synergized by curcumin treatment in vitro. Molecular Biotechnology. 39(1):39-48 [Springer publishing group]

  73. P. Gopinath and Ghosh SS (2007). Monitoring green fluorescent protein for functional delivery of E. coli cytosine deaminase suicide gene and the effect of curcumin in vitro. Gene Therapy and Molecular Biology. 11: 219-228 [GTMB publishing group]

  74. Nellimoottil T. T, Rao P. N, Ghosh SS, Chattopadhyay A. (2007). Evaporation-induced patterns from droplets containing motile and nonmotile bacteria. Langmuir. 23 (17), 8655-8 [ACS publishing group]

  75. Nellimoottil T. T, Rao P. N, Ghosh SS, Chattopadhyay A. (2007).Reply to comment on evaporation-induced patterns from droplets containing motile and nonmotile bacteria. Langmuir. 23:11942-11942 [ACS publishing group]

  76. Ghosh SS, P. Gopinath and Ramesh A. (2006). Adenoviral vectors: A promising tool for gene therapy. Applied Biochemistry and Biotechnology.133 (1), 9-29 [Springer publishing group]

  77. Gogoi S. K, P. Gopinath, Paul A, Ramesh A, Ghosh SS and Chattopadhyay A. (2006). Green fluorescent protein-expressing Escherichia coli as a model system for investigating the antimicrobial activities of silver nanoparticles. Langmuir. 22, 9322-9328 [ACS publishing group]

  78. Ghosh SS, Lu Y, Lee W, Wang X, Guha C, Roy-Chowdhury J and Roy-Chowdhury N (2005). Role of cysteine residues in the function of human UDP Glucuronosyltransferase isoform 1A1 (UGT1A1),Biochemical Journal 15; 392(Pt 3):685-92.

  79. Guha C,Ghosh SS, Lee SW, Roy-Chowdhury N and Roy-Chowdhury J (2004). Hepatocyte transplantation and liver-directed gene therapy, Molecular Pathogenesis of Cholestasis, 340-360.

  80. Kren BT, Ghosh SS, Linehan C.L, Roy Chowdhury N, Hackett P.B, RoyChowdhury J, Steer C.J, (2003). Hepatocyte-targeted delivery of Sleeping Beautymediates efficient gene transfer in vivo,Gene Therapy and Molecular biology, 7, 229-238.

  81. Strayer D.S, Goldstein H, Cordelier P, Ghosh SS, Strayer M.S, Pettoello- Mantovani M, Roy Chowdhury J (2003). Using new gene delivery systems to advanceHIV gene therapy,Clinical and Applied Immunology Reviews, 3 (4-5), 247-259.

  82. Guha C, Shah SJ, Ghosh SS, Roy Chowdhury N and Roy Chowdhury J (2003). Molecular therapies for viral hepatitis,BioDrugs.17 (2): 81-91.

  83. Ghosh SS, SW Lee, Roy Chowdhury J and Roy Chowdhury N (2003) Critical role of cysteine residues in human UGT1A1 in bilirubin glucuronidation, Hepatology38, 384.

  84. N.R.Thummala, Ghosh SS, Lee S.W, Horwitz M.S, Reddy B, Davidson A, Roy Chowdhury J, Roy Chowdhury N (2002) A non-immunogeneic adenoviral vector, co-expressing CTLA4Ig and bilirubin-uridine- diphospoglucuronate-glucuronosyltransferase permits long-term, repeatable transgene expression in the GUNN rat model of Crigler-Najjar syndrome, Gene Therapy. 9(15): 981-90.

  85. Sappal B.S, Ghosh SS, Shneider B, Kadakol A, Roy Chowdhury J and Roy Chowdhury N (2002). A novel intronic mutation results in the use of a cryptic splice acceptor site within the coding region of UGT1A1, causing Crigler-Najjar Syndrome Type 1, Molecular Genetics and Metabolism, 75(2): 134-42.

  86. Strayer D.S, Branco F, Zern M.A, Yam P, Calarota S.A, Nichols C.N, Zaia J.A, Rossi J, H.Li, Parashar B, Ghosh SS, and RoyChowdhury J (2002). Durability of Transgene Expression and Vector Integration: Recombinant SV40-Derived Gene Therapy Vectors, Molecular Therapy, 6(2): 227.

  87. Ghosh SS, Sappal B.S, G.V.Kalpana, S.W.Lee, Roy Chowdhury J and RoyChowdhury N (2001).Homodimerization of human- uridinediphosphoglucuronateglucuronosyl-transferase-1 (UGT1A1) and its functional implications,Journal of Biological Chemistry, 276 (45): 42108-15.

  88. Guha C, Deb N.J, Sappal B.S, Ghosh S.S, Roy Chowdhury N and Roy Chowdhury J (2001). Amplification of Engrafted Hepatocytes by Preparative Manipulation of the Host Liver, Artificial Organs. 25 (7): 522-528

  89. Kawashita Y, Caton M, Ghosh SS, Takahashi M, Roy Chowdhury N, Okuyama T, Guha C, C.B Whitley, Strayer D.S, Roy Chowdhury J (2001). Recombinant SV40 vector-mediated transfer of beta-glucuronidase gene into mucopolysaccharidosis type VII mice, Molecular Therapy, 3, 5

  90. Ghosh SS, Takahashi M, Parashar B, N.R.Thummala, Roy Chowdhury N and Roy Chowdhury J (2000). Liver directed gene therapy; promises, problems and prospects at the turn of the century,The Journal of Hepatology. 32: 238-252.

  91. Roy Chowdhury N, Kadakol A, Sappal B.S, Ghosh SS, Lee SW and Roy Chowdhury J (2001).Gene therapy for hyperbilirubinemic conditions, Journal of Perinatology, 21: S114-118.

  92. N Thummala, Ghosh SS, Lee S, M Caton, A Davidson, B Reddy (2001). A non-immunogenic adenoviral vector, coexpressing CTLA4Ig and bilurubin-UDP-glucuronosyltransferase (UGT1A1) permits long-term, repeatable transgene expression in the Gunn rat model of Crigler-Najjar syndrome type 1,  Hepatology 34 (4), 357A-356A.

  93. Kadakol A, Sappal B.S, Ghosh SS, Lowenheim M, Chowdhury A, Chowdhury S, Santra A, I.M. Arias, Roy Chowdhury J, and Roy Chowdhury N (2001). Interaction of. Crigler-Najjar-type and Gilbert’s-type mutations causes intermediate degrees of hyperbilirubinemia and may cause kernicterus,Journal of Medical Genetics. 38(4): 244-249.

  94. Kadakol A, Sappal B.S, Ghosh SS, Roy Chowdhury J and Roy Chowdhury N. (2000).  Inherited Disorders of bilirubin Metabolism Gastroenterology Today. 4, 1, 2000.

  95. Parashar B, Ghosh SS, Takahasi M, Roy Chowdhury N, Roy Chowdhury J (2000). Comparison of currently existing methods of gene transfer to the liver and prospects of liver-directed gene therapy, Gastroenterology Today. Vol.1V, 1, 2000.

  96. Kadakol A, Ghosh SS, Sappal B.S, Sharma G., Roy Chowdhury J and Roy Chowdhury N(2000).Genetic Lesions of Bilirubin Uridinediphospho-glucuronateGlucuronosyltransferaseCausing Crigler-Najjar and Gilbert's Syndromes: Correlation of Genotype to Phenotype,Human Mutation. 16: 297-306.

  97. Kadakol A, Takahashi M, Parashar B, Guha C, Ghosh S.S, Roy Chowdhury N and Roy Chowdhury J (1999). Hepatocyte transplantation at the turn of the century Prospects and remaining hurdles,Organ Biology. 6: 4, 43-51.

  98. Ghosh S.S, Kadakol A, Sauter B.V, Takahashi M, Roy Chowdhury N and Roy Chowdhury J (1999). Recombinant Simian virus 40 vectors integrate into host genome, and permit efficient, long-term and repeatable gene transfer to the liver in vivo,Hepatology, 30:298A, 1999.

  99. Ghosh SS, Mukherjee S, Adhya S. Molecular karyotyping of Indian Leishmaniadonovani strains using nuclear DNA probes (1998), Journal of Biosciences. 23: 247- 254.

  100. Chakraborti S, Michael J.R, Gurtner GH, Ghosh SS, Dutta G, Merker A (1993). Role of a membrane-associated serine esterase in the oxidant activation of phospholipase A2 by t-butyl hydroperoxide. Biochemical Journal, 292: 585- 589.

  101. MD.Q.Hassan, Ghosh A, Ghosh SS, Gupta M, Basu D, Mallik KKandAdhya S (1993). Enzymatic amplification of mini-exon-derived RNA gene spacers of Leishmaniadonovani; primers and probes for DNA diagnosis. Parasitology, 107: 509- 517.

       Ph.D. Completed:

SL. No.

Name

Title of the Thesis

Period

Current Address

From

To

1.

Dr. P. Gopinath

Prodrug gene therapy vectors in combination therapies

January 2004

August

2008

Associate Professor, Department of Biotechnology, IIT Roorkee, joined 2011

2.

Dr. Pallab  Sanpui

 

(Joint with Prof. A. Chattopadhyay)

Therapeutic implications of polymer -metal nanoparticle composites

July

2005

January

2011

Assistant Professor,

BITS Pilani (Dubai)

since 2017

3.

Dr. Vinod Kr. Yata

Suicide Enzymes: Purification, Characterization and Encapsulation for Therapeutic Implications

January

2008

March

2012

Assistant Professor, Department of Biotechnology, NIT Jalandhar, since 2012

4.

Dr. Shilpa Sharma

 

(Joint with Prof. A. Chattopadhyay)

Metal nanoparticles and nanocomposites as antimicrobial and anticancer agents

July 2008

March

2013

Assistant Professor, Division of Biotechnology, Netaji Subhas Institute of

Technology (NSIT), Delhi, since 2014

5.

 

 

Dr. Amit Jaiswal

 

(Joint with Prof. A. Chattopadhyay)

Fluorescent Nanomaterials for Biological Applications

 

 

July 2010

 

 

September

2013

 

Assistant Professor,

School of Basic Sciences,

IIT Mandi, joined 2014

6.

Dr. V. Kohila

Genetic engineering approaches to alter substrate specificity of suicide genes

July 2008

March

2014

Assistant Professor, Department of Biotechnology, NIT Warangal, joined 2013

7.

Dr. Subhamoy Banerjee

Signaling molecules in combination therapy

January

2009

April

2014

Assistant Professor,

School of life sciences, B.S. Abdur Rahman University, joined 2016

8.

Dr. S. Chokalingam

Molecular  approaches for development of efficient  suicide gene therapy

July 2008

June

2014

Postdoctoral fellow, 

University of Bristol, since 2014

9.

 Dr. Nidhi Chaubey

Molecular Cloning, Expression, Purification and Functional Implications of Recombinant Cytokines

January 2009

June

2014

 -

10.

Dr. Mithun Chakraborty

 

(Joint with Prof. P. Goswami)

Molecular Characterization of Broad Substrate Specific Alcohol Oxidase from Aspergillus terreus  MTCC 6324

July 2009

August 2014

Assistant Professor,

Department of Biotechnology

Chandigarh University, Mohali, joined 2016

11.

Dr. Amaresh Kr. Sahoo

 

(Joint with Prof. A. Chattopadhyay) 

Drug Based Nanocomposites in Biological Applications

July 2009

March

2015

Assistant Professor,

Indian Institute of Information Technology (IIIT) Allahabad, joined 2016

12.

Dr. Archita Ghoshal

Cell Surface Targeted Recombinant Signaling Molecules

July 2011

June

2016

Postdoctoral fellow, Penn State College of Medicine, Hershey, USA, since Jan 2017

13.

Dr. N. Sharmila

 

(Joint with Prof. L. Sahoo)
 

Functional Characterizations of Plant Uracil Phosphoribosyltransferase and Phytaspase for their Potential in Cancer Therapy

July 2011

April

2017

Assistant Professor,

Department of Biotechnology , NIT Raipur, joined since July 2017

    Current Ph.D. students:

SL. No.

Name

Title of the Thesis or Research Area

Period

Status/ Category

From

To

1.

Asif Raza

Cell Communication in Cancer Gene Therapy

July 2012

June 2017

(synopsis seminar)

Regular

2.

Upashi Goswami 

(Joint with Prof. A. Chattopadhyay)

Nanocarriers for targeted delivery

 

July 2012

Ongoing

Regular

(Project sponsored)

3.

Bandhan Chatterjee 

(Joint with Prof. A. Chattopadhyay)

Nanocarriers for drug delivery

July 2012

Ongoing

Regular

  1.  

Neha Arora

Signaling pathways in Cancer

July 2013

Ongoing

Regular

  1.  

Deepanjalee Dutta 

(Joint with Prof. A. Chattopadhyay)

Nanoclusters in theranostics

July 2013

Ongoing

Regular

  1.  

Ujjwol Barman 

(Joint with Prof. Roy P. Pailey)

FET based Sensors

July 2013

Ongoing

Regular

  1.  

Bidkar Anil Parsram

 

Cancer Gene therapy

July 2014

Ongoing

Regular

  1.  

Dr. Madhumita Das 

(Joint with Prof. A. Chattopadhyay)

Nanobiotechnology in medical devices

July

2014

Ongoing

Regular

  1.  

Tammana Bhuyan 

(Joint with Dr. D. Bandhyopadhyay)

Controlled movements of Biomotors and  Microfluidics

July 2014

Ongoing

Regular

  1.  

Srirupa Bhattacharyya

Protein Therapeutics

July 2015

Ongoing

Regular

  1.  

Rajiv Shome

Cancer Gene Therapy

July 2016

-

Regular

  1.  

Anitha T Simon

(Joint with Prof. A. Chattopadhyay)

Nanocarriers for drug delivery

July 2016

-

Regular

(Project sponsored)

  1.  

Muktashree Saha

Cancer Gene Therapy

July 2017

-

Regular

      M.Tech. Students: 

     Number of students completed their M.Tech. Projects: 06 

      B.Tech. Students:

     Number of students completed B.Tech. projects: 26
     Current student: 01
 


 
Indian Institute of Technology Guwahati 2013 Department of Biotechnology
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