Dr. Ranjan Tamuli

Associate Professor
Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati
Guwahati-781 039, Assam, India.
Email: ranjantamuli[at]iitg.ernet.in
Tel: +91 361 2582220
Fax: +91 361 2582249

 

ACADEMIC:

Professional Track
 

25 January 2014-present: Associate Professor: Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati.

26 December 2008-24 January 2014: Assistant Professor: Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati.

 

Research Interest:

Calcium signaling, Fungal genetics, DNA repair

EDUCATION

2008: Ph.D., Centre for Cellular and Molecular Biology, Hyderabad.
2002: M.Sc., Molecular Biology and Biotechnology, Tezpur University, First Class with Distinction.
2000: B.Sc., Zoology Major, Dibrugarh University, First Class.
1997: H.S., Science, Dhemaji College, AHSEC, First Division.
1995: H.S.L.C, Dhemaji Higher Secondary School, SEBA, First Division.

Additional Research Experiences:

December 2013 to- December 2014: IUSSTF Research Fellow at the University of California Riverside, USA.

2008 (March to December): Research scholar, Department of Environmental and Occupational Health, Florida International University, Miami, USA.

 2007 (November)-2008 (March): Research Scholar, Department of Biochemistry, University of Florida, Gainesville, USA.

Awards and Honors:

 IUSSTF Research Fellow: Awarded Indo-US Research Fellowship 2013 by the Indo-US Science and Technology Forum (IUSSTF) for research (December 9, 2013 to – December 5, 2014) at the University of California Riverside, USA.

TEACHING EXPERIENCES (DECEMBER 2008-PRESENT):

Ph. D. Course:

  • BT623: Research Methodology

M. Tech. (PG) Courses:

  • BT502: Quantitative Biology
  • BT503: Advanced Genetic Engineering
  • BT520: Applied Biology and Bio-engineering Lab
  • BT600: M. Tech. Seminar
B.Tech (UG) Courses:
  • BT101: Modern Biology
  • BT208: Molecular Biology and Genetic Engineering
  • BT303: Immunology
  • BT307: Environmental Biotechnology

 

Scientist:

 

Dr. Anand Tiwari (DST-Young Scientist)

2015-present

Research Interest: Molecular physiology of heavy metal transport, bioremediation, inorganic nanoparticle synthesis, cell polarity in morphogenesis, live-cell imaging

Email id:anand.bio[at]iitg.ernet.in; anand.bio[at]gmail.com

 

Ph. D. Students:

 

Ananya Barman (Graduate Student)

2011-present

Research Interest: Investigation of calcium signaling pathways mediated by phospholipase C-1 (PLC-1), secretory phospholipase A2 (sPLA2), and calcium proton exchanger-1 (CPE-1) in N. crassa.

Email id: b.ananya[at]iitg.ernet.in

Dibakar Gohain (Graduate Student)

2013-present

Research interest: Molecular mechanism and genetics of Neuronal calcium sensor-1 (NCS-1).

Email id: g.dibakar[at]iitg.ernet.in

Ajeet Kumar (Graduate Student)

2014-present

Research Interest: Understanding the functions of calcineurin in N. crassa.

Email id: ajeetkumar.2014[at]iitg.ernet.in

Avishek Roy (Graduate Student)

2015-present

Research interest: Understanding the Molecular mechanism and genetics of calcineurin-B subunit gene in N. crassa.

Email id: r.avishek[at]iitg.ernet.in

Christy Noche K. Marak (Graduate Student)

2015-present

Research Interest: Molecular mechanism of calcium/calmodulin-dependent kinases in N. crassa.

Email id: c.marak[at]iitg.ernet.in

 

Darshana Baruah (Graduate Student)

2016-present

Research Interest: Understanding molecular mechanisms of plc-1, splA2, and cpe-1 in N. crassa.

Email id:darshana[at]iitg.ernet.in

 

 

Serena Ngilmei D (Graduate Student)

2017-present

Research Interest: Molecular analysis of a of a zinc transporter in N. crassa.

Email id:serena[at]iitg.ernet.in

 

M. Tech. Student:

Pallavi Das (M.Tech Project Student)

2017-present

Research Interest: Understanding the molecular mechanism of  trm-9 in N. crassa.

Email id:pallavidas[at]iitg.ernet.in

ALUMNI

Dr. Vijya Laxmi (Graduate Student), Year of completion: 2017

 Current position: Assistant Professor, Department of Agricultural Sciences, SGT University, Shree Guru Gobind Singh Tricentenary, Gurugram, Haryana 122 505

Dr. Ravi kumar ( Graduate Student), Year of completion: 2014

Dr. Rekha Deka (Graduate Student), Year of completion: 2014 

Serena Ngilmei D (M.Tech Project Student)
Period : 2016-2017

Manju Nagar (M.Tech Project Student)
Period : 2015-2016, joined as a JRF in the Department of Chemical Engineering,

Indian Institute of Science Education and Research, Bhopal 462 006

Vishakha (M.Tech Project Student)
Period : 2013-2014

Kumar Jagadeesh (M.Tech Project Student)
Period : 2012-2013

Dhruv Aditya Srivastava (M. Tech Project Student)
Period : 2011 - 2012, joined as a Ph. D. student at the Agricultural Research Organization, Volcani Center,

The Hebrew University of Jerusalem, Israel

 

Ravi Gedela ( M. Tech Project Student)
Period: 2010-2011, joined as a Lecturer, Department of Biosciences,

Rajiv Gandhi University of Knowledge Technologies, Nuzvid 521 201 

 

 

PROJECT STUDENTS, BTP, AND OTHERS:

6 JRFs, 8 BTPs, and 19 summer trainees (both internal and external) had undergone research training in the lab during the period from 2009 to 2017;

a few students perused higher studies at various institutions including Indian Institute of Management, Rohtak,  University of Wisconsin, Madison etc.

 

 

PUBLICATIONS

  1. Barman A, Tamuli R (2017) The pleiotropic vegetative and sexual development phenotypes of Neurospora crassa arise from double mutants of the calcium signaling genes plc-1, splA2, and cpe-1. Current Genetics DOI 10.1007/s00294-017-0682-y [Epub ahead of print].

  2. Laxmi V, Tamuli R (2017) The calmodulin gene in Neurospora crassa is required for normal vegetative growth, ultraviolet survival, and sexual development. Archives of Microbiology 199(4):531-542.

  3. Gohain D, Deka R, Tamuli R (2016) Identification of critical amino acid residues and functional conservation of the Neurospora crassa and Rattus norvegicus orthologues of neuronal calcium sensor-1. Genetica 144(6):665-674.

  4. Tamuli R, Deka R, Borkovich KA (2016) Calcineurin subunits A and B interact to regulate growth and asexual and sexual development in Neurospora crassa. PLoS One 11(3): e0151867. doi: 10.1371/journal.pone.0151867.

  5.  Tamuli R (2016) Neurospora: A scientific journey by the orange mold since 1843. North East Bioline 1: 8-9.

  6.  Deka R, Ghosh A, Tamuli R, Borkovich KA (2016) Heterotrimeric G Proteins. In H. Drik (ed.), pp 119-144, The mycota III. Springer-Verlag, Berlin, Germany. DOI 10.1007/978-3-319-27790-5_7.

  7. Laxmi.V and Tamuli.R (2015). The Neurospora crassa cmd, trm-9, and nca-2 genes play a role in growth, development, and survival in stress conditions. Genomics and Applied Biology. 6:1-8.

  8. Barman A. and Tamuli R. (2015). Multiple cellular roles of Neurospora crassa plc-1, splA2 and cpe-1 in regulation of cytosolic free calcium, carotenoid accumulation, stress responses, and acquisition of thermotolerance. Journal of Microbiology DOI 10.1007/s12275-015-4465-1.

  9.  Kumar R. and Tamuli R. (2014). Calcium/calmodulin-dependent kinases are involved in growth, thermotolerance, oxidative stress survival, and fertility in Neurospora crassa. Archives of Microbiology 196: 295-305.

  10. Deka R. and Tamuli R. (2013). Neurospora crassa ncs-1, mid-1 and nca-2 double mutant phenotypes suggest diverse interaction among three Ca2+ regulating gene products. Journal of Genetics. 92: 559-563.

  11.  Tamuli R., Kumar R., Srivastava D. A. and Deka R. (2013). Calcium signaling. In Neurospora: Genomics and Molecular Biology (ed. D. P. Kasbekar and K. McCluskey), pp. 35-57. Caister Academic Press, Norfolk, UK.

  12.  Deka, R., Kumar, R. and Tamuli, R. (2011). Neurospora crassa homologue of Neuronal Calcium Sensor-1 has a role in growth, calcium stress tolerance, and ultraviolet survival. Genetica 139: 885-894.

  13.  Tamuli, R., Kumar R. and Deka, R. (2011). Cellular roles of neuronal calcium sensor-1 and calcium/calmodulin-dependent kinases in fungi. J. Basic Microbio. 51: 120-128.

  14. Goel M, Tamuli R. (2011). RPL10 (ribosomal protein L10). Atlas Genet Cytogenet Oncol Haematol. 15: 425-427.

  15.  Mandrawalia R, Tamuli R. (2010). AMOT (angiomotin). Atlas Genet Cytogenet Oncol Haematol.14:1121-1123.

  16. Deka R, Tamuli R. (2010). CDT1 (chromatin licensing and DNA replication factor 1). Atlas Genet Cytogenet Oncol Haematol.14:812-814.

  17.  Singh PK, Tamuli R. (2010). CAPG (capping protein (actin filament), gelsolin-like). Atlas Genet Cytogenet Oncol Haematol. 14:809-811.

  18. Bhattacharyya, M., Tamuli, R. (2010). SEMA3B (sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3B). Atlas Genet Cytogenet Oncol Haematol. 14:662-664.

  19. Sinha, N., Tamuli, R. (2010). RBBP7 (retinoblastoma binding protein 7). Atlas Genet Cytogenet Oncol Haematol. 14:578-580.

  20. Mandrawalia, R., Tamuli, R. (2010). KCMF1 (potassium channel modulatory factor 1). Atlas Genet Cytogenet Oncol Haematol.14:560-561.

  21. Tamuli, R. (2010). Genome Defense Mechanisms in Neurospora and Associated Specialized Proteins. Journal of Proteins and Proteomics 1: 15-23.

  22. Roy, D., Tamuli, R. (2009). NRF1 (nuclear respiratory factor 1). Atlas Genet Cytogenet Oncol Haematol.13:861-863.

  23. Tamuli, R. and Kasbekar, D. P. (2008). Dominant suppression of repeat-induced point mutation (RIP) in Neurospora crassa by a variant catalytic subunit of DNA polymerase zeta (Pol z). Genetics 178: 1169-1176.

  24. Tamuli, R., Ravindran, C. and Kasbekar, D. P. (2006). Translesion DNA polymerases Pol z, Pol h, Pol i , Pol k and Rev1 are not essential for repeat-induced point mutation (RIP) in Neurospora crassa. J. Biosci. 31: 557-564.

  25. Bhat, A., Tamuli, R., and Kasbekar, D. P. (2004). Genetic transformation of Neurospora tetrasperma, demonstration of repeat-induced point mutation (RIP) in self-crosses and a screen for recessive RIP-defective mutants. Genetics 167: 1155-1164.

Conference presentations:

  1. Kumar A, Roy A, Gohain D, and Tamuli R (2017) Calcineurin- a serine/threonine protein phosphatase and its role in calcium signaling. Research Conclave, IIT Guwahati 17-20 March.

  2. Marak K CN and Tamuli R (2017) Understanding the molecular mechanism of calmodulin and calcium/calmodulin-dependent kinases in Neurospora crassa. Research Conclave, IIT Guwahati 17-20 March.

  3. Ngiimei DS and Tamuli R (2017) Cellular role of a Zinc transporter in Neurospora crassa. Research Conclave, IIT Guwahati 17-20 March.

  4. Gohain D and Tamuli R (2016) Understanding the functions of neuronal calcium sensor-1 (NCS-1) of Neurospora crassa and its rat orthologue. XL All India Cell Biology Conference & International Symposium on Functional Genomics and Epigenomics, Galav Sabhagar, Jiwaji University, Gwalior, November 17-19.

  5. Roy A and Tamuli R (2016) Studies on the molecular mechanism of Calcineurin regulatory subunit in Neurospora crassa.  XL All India Cell Biology Conference & International Symposium on Functional Genomics and Epigenomics, Galav Sabhagar, Jiwaji University, Gwalior, November 17-19.

  6. Barman.A , Nagar.M, Kumar.A and Tamuli.R (2016). Calcium signaling and regulation of cell function in Neurospora crassa. Research Conclave, IIT Guwahati, India, 18-20 March, 2016.

  7. Gohain.D, Roy.A and Tamuli.R (2016). Neurospora crassa as a model organism for the functional investigation of neuronal calcium sensor-1(NCS-1) from higher eukaryotes. Research Conclave, IIT Guwahati, India, 18-20 March, 2016.

  8. Tiwari.A and Tamuli.R (2016). Immortal fungal biomass for eco-friendly and scalable nanofactories. Research Conclave, IIT Guwahati, India, 18-20 March, 2016.

  9. Gohain.D and Tamuli.R (2015). Involvement of rat NCS-1 in ultraviolet light induced DNA damage repair process. 9th International conference on Yeast Biology , Jadavpur University, Kolkata, India, 9-12 December, 2015.

  10. Kumar.A and Tamuli.R (2015). A novel approach to identify critical amino acid residues for calcineurin -A of Neurospora crassa. 9th International conference on Yeast Biology , Jadavpur University, Kolkata, India, 9-12 December, 2015.

  11. Nagar.M and Tamuli.R (2015). Molecular characterization of phospholipase c-δ subtype genes from the model filamentous fungus Neurospora crassa. 9th International conference on Yeast Biology , Jadavpur University, Kolkata, India, 9-12 December, 2015.

  12. Roy.A and Tamuli.R (2015). Studies on the role of calcium signaling genes in regulating cytosolic free calcium concentration in Neurospora crassa. 9th International conference on Yeast Biology , Jadavpur University, Kolkata, India, 9-12 December, 2015.

  13. Barman, A., Gohain, D. and Tamuli, R. (2015). Functional analysis of calcium signaling genes and a novel approach to study direct protein-protein interaction in Neurospora crassa. Research Conclave, IIT Guwahati, India, 23rd March.

  14. Laxmi V., Kumar A., Tamuli R. (2015). Studies on essential calcium signaling genes calmodulin and calcineurin in Neurospora crassa. Research Conclave, IIT Guwahati, India, 23rd March.

  15. Barman, A., Gohain, D., Laxmi, V., Deka, R., Kumar, R. and Tamuli, R. (2015). Cellular functions of calcium signaling machinery in the model filamentous fungus Neurospora crassa, Molecular intricacies of Plant-pathogenic micro-organisms, Tezpur University, Tezpur, India, 21-22 February.

  16. Laxmi V., Tamuli R. (2015). Expression studies of important calcium signaling genes in Neurospora crassa. IBS-2015, National Symposium on Biophysics and Golden Jubilee Meeting of Indian Biophysical Society, Jamia Millia Islamia, New Delhi 10025, India, February 14-17, 2015.

  17. Barman, A., Gohain, D. and Tamuli, R. (2014). Reactive oxygen species and cell signaling in Neurospora crassa, International conference on disease biology and therapeutics, Institute of Advanced study in Science and Technology, Guwahati, India, 3-5 December.

  18. Gohain, D., Barman, A. and Tamuli, R. (2014). Calcineurin and its role in drug resistance in pathogenic fungi, Institute of Advanced study in Science and Technology, Guwahati, India, 3-5 December.

  19. Laxmi V., Tamuli R. (2014). Studies of calcium signaling pathway mediated by calmodulin and related proteins in Neurospora crassa. ICDBT‐2014, International Conference on Disease Biology and Therapeutics-2014, Institute of Advanced Study in Science and Technology, Guwahati, India, December 3-5, 2014.

  20. Tamuli R. Borkovich, K., (2014). Studies on essential calcium-signaling genes using a conditional promoter-based system. Riverside Postdoctoral Association Symposium, University of California Riverside, USA, 23-24-September.

  21. Tamuli R., Deka R., and Borkovich K.A. (2014). A cytogenetic approach to study calcium signaling genes of Neurospora crassa. Neurospora 2014, Asilomar, CA 93950-3704, 6-9 March.

  22. Deka R., Kumar R., Laxmi V., Barman A., Vishakha, Gohain D., Borpujari M., Borah R., Singh Kh.S., and Tamuli R (2013). Calcium signaling in Neurospora crassa. 8th International Conference on Yeast Biology - Yeast 2013, CSIR-Institute of Microbial Technology (CSIR-IMTECH), Chandigarh 160036, India, 4 - 7 December, 2013.

  23. Barman, A. and Tamuli, R. (2013). Role of Ca2+/H+ exchanger and secretory phospholipase A2 in regulating Ca2+ homeostasis in Neurospora crassa, 8th international conference on Yeast biology, Institute of Microbial Technology, Chandigarh, India, 27-30 November.

  24. Gohain, D., Deka, R. and Tamuli, R. (2013). Studies on the effect of the post-translational modifications of Neurospora crassa homologue of neuronal calcium sensor-1 in various cell functions, 8th international conference on Yeast biology, Institute of Microbial Technology, Chandigarh, India, 27-30 November.

  25. Laxmi V., and Tamuli R. (2013). Function of calmodulin protein in Neurospora crassa. 8th International Conference on Yeast Biology - Yeast 2013, CSIR-IMTECH, Chandigarh 160036, India, 4 - 7 December.

  26. Vishakha, and Tamuli R. (2013). Role of Calcium signaling genes in carotenoids accumulation in Neurospora crassa. 8th International Conference on Yeast Biology - Yeast 2013, CSIR-IMTECH, Chandigarh 160036, India, 4 - 7 December.

  27. Laxmi, V. and Tamuli, R. (2012). Cellular roles of calmodulin in Neurospora crassa, 4th Asian chromatin meeting on epigenetic mechanisms in development and disease, Centre for Cellular and Molecular Biology, Hyderabad, India, 22-24 November.

  28. Kumar, R. and Tamuli, R. (2012). Involvement of the Neurospora crassa Ca2+/ CaM-dependent protein kinases in full fertility, survival in exposure to heat shock and oxidative stress, 4th Asian chromatin meeting on epigenetic mechanisms in development and disease, Centre for Cellular and Molecular Biology, Hyderabad, India, 22-24 November.

  29. Jagadeesh, K. and Tamuli, R. (2012). Analysis of mitochondrial carrier proteins from Bombyx mori and Neurospora crassa, International consultative meeting on seribiotechnology (ICMS-2012), Institute of Bioresearches and Sustainable Development (IBSD), Takyelpat Institutional Area, Imphal-795001, Manipur, India, 5-7 December.

  30. Barman, A. and Tamuli, R. (2012). Comparative analysis of cell signaling pathways and methylation patterns in Bombyx mori and Neurospora crassa, International consultative meeting on seribiotechnology (ICMS-2012), Institute of Bioresearches and Sustainable Development (IBSD), Takyelpat Institutional Area, Imphal-795001, Manipur, India, 5-7 December.

  31. Deka, R., Kumar, R., Gedela, R., Sarma, U., Srivastava, D.A., and Tamuli, R. (2011).Calcium signaling in Neurospora crassa. Cell Signaling Networks 2011, Merida, Mexico, 22-27 October.

  32. Tamuli, R., Srivastava, D.A., Kumar, R., Deka, R., and Mahor, D. (2011). Genetics of calcium signaling machinery in Neurospora crassa. Comparative genomics of eukaryotic microorganisms: understanding the complexity of diversity, Sant Feliu de Guixols, Spain, 15 - 20 October.

  33. Deka, R., Kumar, R., Gedela, R., Tamuli, R., (2011). Investigating cellular functions of the calcium signaling genes in Neurospora crassa. World Congress on Biotechnology-2011, Hyderabad, India, 21-23 March.

  34. Deka R, Tamuli R (2011) Cellular roles of the Neurospora crassa neuronal calcium sensor-1 homologue. World Congress on Biotechnology-2011, Hyderabad, India 21-23 March.

  35. Kumar R, Tamuli R (2011) Calcium signaling proteins are essential for full fertility in Neurospora crassa. World Congress on Biotechnology-2011, Hyderabad, India 21-23 March.

  36. Deka, R., Kumar, R., and Tamuli, R. (2010). Calcium signaling genes in Neurospora crassa. Neurospora 2010 Meeting, Asilomar, USA, 8-11 April.

  37. Deka, R., Kumar, R., Mandrawalia, R., and Tamuli, R. (2009). Genetic analysis of calcium signaling genes in Neurospora crassa. International Workshop on Biology of Yeasts and Filamentous Fungi, Indo-U.S. Science and Technology Forum, Centre for Cellular and Molecular Biology, Hyderabad, India, 11-14 December.

  38. Tamuli, R., Singh, P., Iyer, S., Ramakrishnan, M., Vyas, M. and Kasbekar, D. P. (2007). Two kinds of dominant RIP suppressors in Neurospora crassa. International Symposium on Chromosomes to Genome, Centre for Cellular and Molecular Biology, Hyderabad, India, 3-5 July.

  39. Tamuli, R., and Kasbekar, D. P. (2006). Role of repair-related DNA polymerases in RIP. Association for the Promotion of DNA Fingerprinting and other Technologies (ADNAT) Conference, Centre for Cellular and Molecular Biology, Hyderabad, India, 23-24 February.

  40. Tamuli, R., and Kasbekar, D. P. (2006). Role of translesion DNA polymerases in RIP. XXIX All India Cell Biology Conference, Industrial Toxicology Research Centre, Lucknow, India, 17-20 January.

  41. Tamuli, R., and Kasbekar, D. P. (2005). Analysis of the dominant RIP suppressor of the Adiopodoume strain of Neurospora crassa. International Conference on the Biology of Yeasts, Indian Institute of Science, Bangalore, India, 27-29 September.

  42. Tamuli, R., and Kasbekar, D. P. (2005). Genetic and molecular basis of the dominant RIP suppressor of the Adiopodoume strain of Neurospora crassa. XXIII Fungal Genetics Conference, Asilomar, USA, 15-20 March.

 

LAB PROTOCOLS:

  1. Neurospora protocol guide (FGSC)
  2. Stanford Neurospora Methods
  3. Media and stocks

 

RESEARCH:

What we do?

We are interested to understand the molecular mechanism of calcium signaling pathway using the model filamentous fungus Neurospora crassa. Calcium ion is a universal second messenger molecule that impacts almost all cell processes in eukaryotes. We hope to extend our research to understand the role of calcium signaling in memory, learning, and other related areas in future.


Ongoing Projects:

  1. Functional characterization and role of TMSF2 , a NRAMP family member , in the life cycle of Neurospora crassa (DST-SERB Young Scientist) 2016-2018, Dr. Anand Tiwari.
  2. Molecular investigation of epigenetic modifications caused by environmental pollution using Neurospora crassa as a model system, supported by the Department of Biotechnology (DBT), India, 2013.


Completed Projects:

  1. Studies on the cellular roles of calcium signaling proteins in Neurospora crassa, supported by the Department of Biotechnology (DBT), India, 2011.
  2. Functional analysis of calcium signaling proteins in Neurospora crassa, supported by the Department of Science and Technology (DST), India, 2010-2013.
  3. Functional analysis of translasion DNA polymerase Pol eta (h), Pol iota (i), and Pol kappa (k) in Neurospora crassa, IIT Guwahati Start-up grant, 2009-2011.